Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608118
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Anticonvulsant Principle Isolation of the Marine Diatom Skeletonema marinoi

T Brillatz
1   School of Pharmacy, University of Geneva, University of Lausanne, rue Michel Servet 1, CH-1211, Geneva 4, Switzerland
,
C Lauritano
2   Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Napoli, Italy
,
M Jacmin
3   Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 6, avenue du Swing, 4367 Belvaux, Luxembourg, Luxembourg
,
S Khamma
1   School of Pharmacy, University of Geneva, University of Lausanne, rue Michel Servet 1, CH-1211, Geneva 4, Switzerland
,
L Marcourt
1   School of Pharmacy, University of Geneva, University of Lausanne, rue Michel Servet 1, CH-1211, Geneva 4, Switzerland
,
D Righi
1   School of Pharmacy, University of Geneva, University of Lausanne, rue Michel Servet 1, CH-1211, Geneva 4, Switzerland
,
G Romano
2   Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Napoli, Italy
,
F Esposito
2   Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Napoli, Italy
,
A Ianora
2   Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Napoli, Italy
,
AD Crawford
3   Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 6, avenue du Swing, 4367 Belvaux, Luxembourg, Luxembourg
,
EF Queiroz
1   School of Pharmacy, University of Geneva, University of Lausanne, rue Michel Servet 1, CH-1211, Geneva 4, Switzerland
,
JL Wolfender
1   School of Pharmacy, University of Geneva, University of Lausanne, rue Michel Servet 1, CH-1211, Geneva 4, Switzerland
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 

Neuroscience is in needs of new drugs to overcome risen CNS disorders because of the increasing human life expectancy [1]. Marine organisms constitute an unexplored source compared to terrestrial plants in the search of novel type of anticonvulsant natural products [2]. In the context of a screening of marine diatoms in a zebrafish epilepsy model with seizures induced by the GABAA antagonist pentylenetetrazole, the methanolic extract of Skeletonema marinoi revealed a significant activity. A bioassay-guided fractionation was undertaken by reverse phase flash chromatography after enrichment with vacuum liquid chromatography in order to isolate the bioactive compounds [3]. This rapid approach leads to the identification of the nucleoside inosine as the main active antiepileptic constituent. Inosine is known for its anticonvulsant activities but was never isolated from diatoms. Structures of isolated compounds were elucidated by spectroscopic methods including evaporative light scattering detector (ELSD), nuclear magnetic resonance (NMR) and high resolution mass spectrometry (HRMS). Additionally, the extract of Skeletonema marinoi was submitted to a metabolite profiling by UHPLC-HRMS. It reveals an interesting chemical diversity and different algal metabolites were annotated together with additional other putative compounds.

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[2] Montaser R, Luesch H. Future Med Chem 2011; 3: 1475 – 1489

[3] Challal S, Queiroz EF, Debrus B, Kloeti W, Guillarme D, Gupta MP, Wolfender JL. Planta Med 2015; 81: 1636 – 1643