Phytochemical study of the Limonium leptophyllum plants and isolated substance

From Limonium gmelinii a number of highly effecient medicinal preparations have been obtained possessing anti-inflammatory, antiviral and wound-healing activities with no allergic reactions and cumulative properties. Currently endemic plant Limonium leptophyllum was studied. Preparation of plant material collected in September, 2016 in Almaty region consisted in its drying and refinement to 3 mm. In accordance with the requirements of the State Pharmacopoeia of the Republic of Kazakhstan, the authenticity for the studied type of medicinal plant material was established by the methods of macroscopy and microscopy. Quality indices were esteemed. Identification of various groups of biologically active compounds for substance standardization and assessment of active compounds for their comparative biological screening was carried out. The results obtained: humidity – 9.59%; total ash – 10.84%; ash, insoluble in 10% HCl – 0.02%; sulphated ash – 10.79%. “Microbiological purity”, “Heavy metals” and “Radionuclides” were established according to the norms for the medicinal plant materials. Pesticides and mycotoxins are not found. Varying the extraction conditions, a rational technological scheme was developed for obtaining a substance from the above-ground part of the plant species under study in the form of a dry extract. Extraction of the studied plant material was carried out twice with 50% ethanol at its ratio with the raw material of 1:6 for 6 hours at room temperature. The substance is a biologically active complex with a high hydrophilicity containing the main classes of biologically active natural compounds in the form of phenolic acids (gallic and ellagic), flavonols (quercetin and myricetin) both in free form and in the form of glycosides, hydrolyzed and condensed tannins, which are dimeric and oligomeric forms of flavan-3-ol. It also includes eight essential α-amino acids, mono- and oligosaccharides, vitamins, microelements and other compounds. For the first time, aglikon apigenin is isolated from this genus.