Diagnostic Yield of Multiplex PCR Method in Cerebrospinal Fluid for the Diagnosis of Purulent Meningitis in Children

Jing-Li Zhao; Chun-Zhen Hua; Yong-Ping Xie; Yan-Xiang Pan; Bo-Fei Hu; Wei-Jian Wang; Xiu He

doi:10.1055/s-0040-1719163

Journal of Pediatric Infectious Diseases, Table of Contents

J Pediatr Infect Dis 2021; 16(01): 012-017
DOI: 10.1055/s-0040-1719163

Original Article

Diagnostic Yield of Multiplex PCR Method in Cerebrospinal Fluid for the Diagnosis of Purulent Meningitis in Children

Jing-Li Zhao

¹Department of Infectious Diseases, National Clinical Research Center for Child Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, P.R. China

²Department of Nephrology, National Clinical Research Center for Child Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, P.R. China

,

Chun-Zhen Hua

¹Department of Infectious Diseases, National Clinical Research Center for Child Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, P.R. China

,

Yong-Ping Xie

¹Department of Infectious Diseases, National Clinical Research Center for Child Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, P.R. China

,

Yan-Xiang Pan

³Department of Clinical Laboratory Center, National Clinical Research Center for Child Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, P.R. China

,

Bo-Fei Hu

¹Department of Infectious Diseases, National Clinical Research Center for Child Health, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, P.R. China

,

Wei-Jian Wang

⁴Department of Research and Development, Health Gene Technologies Co., Ltd., Ningbo, Zhejiang, P.R. China

,

Xiu He

⁵Department of Marketing,r Health Gene Technologies Co., Ltd., Ningbo, Zhejiang, P.R. China

› Author Affiliations

Abstract

Objective To evaluate the diagnostic yield of the multiplex polymerase chain reaction (PCR) method in cerebrospinal fluid (CSF) for the diagnosis of purulent meningitis (PM) in children.

Methods PM was diagnosed according to the European Society for Clinical Microbiology and Infectious Diseases guideline (2016). Patients with PM between May 2015 and October 2018 were included. The multiplex PCR method was used to detect eight common identified bacteria in PM. Its sensitivity and specificity were compared with bacteria culture.

Results A total of 106 cases were enrolled. Pathogenic bacteria were identified in 27 (25.5%) cases by culture and in 37 (34.9%) cases by multiplex PCR assay. The top three bacteria were Streptococcus pneumoniae, Escherichia coli K1, and Streptococcus agalactiae. When using culture as the gold standard, the multiplex PCR assay showed a sensitivity of 100, 88.9, and 75.0% for S. agalactiae, S. pneumoniae, and E. coli K1, respectively, and a specificity of more than 91.3% for all three bacteria. For detectable bacteria, the positive rate of the multiplex PCR assay (36.6%, 37/101) was significantly higher than that of the bacteria culture (21.8%, 22/101). When combining the two methods, etiology was identified in 42.5% (45/106) of the patients.

Conclusion Streptococcus pneumoniae, E. coli K1, and S. agalactiae were the predominant pathogens causing pediatric PM. As a rapid method with high sensitivity and specificity, the multiplex PCR assay in CSF could be used as an adjunctive approach with bacteria culture for the pathogen identification of PM.

Keywords

purulent meningitis - multiplex PCR - pathogen - pediatric - cerebrospinal fluid

Full Text

References

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