Planta Med Lett 2016; 3(04): e87-e90
DOI: 10.1055/s-0042-105296
Letter
Georg Thieme Verlag KG Stuttgart · New York

In Vitro Rapid Multiplication Protocol for Ex Situ Conservation of the Rare, Endemic Medicinal Plant Achyranthes coynei

Mayuri Desai1, Pramod J. Hurkadale1, Vinayak Upadhya2, Lalrinsangi Sailo1, Harsha V. Hegde2, 3, Sandeep R. Pai3
  • 1Department of Pharmaceutical Biotechnology, KLE College of Pharmacy, Nehru Nagar, Belagavi, Karnataka State, India
  • 2Herbal Medicine Division, Regional Medical Research Centre, ICMR, Nehru Nagar, Belagavi, Karnataka State, India
  • 3Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, ICMR, Nehru Nagar, Belagavi, Karnataka State, India
Further Information

Publication History

received 30 December 2015
revised 26 February 2016

accepted 15 March 2016

Publication Date:
28 April 2016 (eFirst)

Abstract

Achyranthes coynei is a rare, endemic species with potential medicinal properties. The present study aimed to develop an efficient in vitro rapid multiplication protocol for A. coynei. It was successfully propagated in vitro using apical shoot tips as explants. A combination of 6-benzylaminopurine (3.0 mg · L−1), thidiazuron (0.5 mg · L−1), and kinetin (0.5 mg · L−1) in MS basal proved best for multiple shoot induction, yielding 4.8 shoots per explant after 60 days of culture. The shoots were rooted with a frequency of 80 % after 30 days of culture on MS agar medium fortified with IBA/NAA (0.5 mg · L−1) before being acclimatized in the greenhouse. The developed protocol will serve as a tool to restore the rare status of the plant and will append its sustainable utilization.