PMIO 2017; 4(02): e52-e58
DOI: 10.1055/s-0043-106857
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Biological Activity of Bunium persicum Essential Oil from Western Himalaya

Iris Stappen1, Nurhayat Tabanca2, 3, Abbas Ali2, David E. Wedge4, Jürgen Wanner5, Velizar Gochev6, Vikas Jaitak7, Brij Lal8, Vijay K. Kaul9, Erich Schmidt1, Leopold Jirovetz1
  • 1Department of Pharmaceutical Chemistry, University of Vienna, Vienna, Austria
  • 2National Center for Natural Products Research, The University of Mississippi, University, MS, USA
  • 3Department of Entomology and Nematology, Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA
  • 4US Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, University of Mississippi, University, MS, USA
  • 5Kurt Kitzing Co., Wallerstein, Germany
  • 6Department of Biochemistry and Microbiology, “Paisii Hilendarski” – University of Plovdiv, Bulgaria
  • 7Biodiversity Division, CSIR – Institute of Himalayan Bioresource Technology, Palampur, India
  • 8Centre for Chemical and Pharmaceutical Sciences, Central University of Punjab, Bathinda, India
  • 9Ex-Scientist of CSIR – Institute of Himalayan Bioresource Technology, Palampur, H.P., India
Further Information

Publication History

received 25 November 2016
revised 08 March 2017

accepted 09 March 2017

Publication Date:
14 July 2017 (online)


The essential oil of Bunium persicum collected from cultivated sources in the cold desert area of Lahaul-Spiti is described for its antimicrobial, larvicidal, and biting deterrent activities. Additionally, odor characterization is given. The chemical composition of the essential oil was analyzed by simultaneous GC-MS and GC-FID. γ-Terpinene and p-cymene were found to be the major compounds. Antibacterial testing by an agar dilution assay revealed low activity of the oil against all tested bacteria. Antifungal activity was evaluated against Candida albicans as well as three species of the strawberry anthracnose causing plant pathogen Colletotrichum. Bunium persicum essential oil demonstrated antifungal activity against all four pathogens. Biting deterrent activity against Aedes aegypti was greater than the solvent control, but significantly lower than DEET. The essential oil of B. persicum exhibited larvicidal activity with an LC50 value of 58.6 ppm against Ae. aegypti larvae.