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Am J Perinatol
DOI: 10.1055/s-0043-1778065
Original Article

Pneumoprotein CC16 in the Umbilical Cord Blood of Preterm Neonates

Dimitrios Rallis
1   Department of Pediatrics, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts
2   Neonatal Intensive Care Unit, University of Ioannina, Faculty of Medicine, Ioannina, Greece
,
Aimilia Eirini Papathanasiou
1   Department of Pediatrics, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts
,
Helen Christou
1   Department of Pediatrics, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts
› Author Affiliations Funding None.
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Abstract

Objective We examined the impact of perinatal factors on cord serum club cell protein (CC16) and the association of CC16 with mechanical ventilation and bronchopulmonary dysplasia (BPD) in preterm neonates.

Study Design A retrospective cohort study including 60 neonates born with gestational age (GA) < 34 weeks. The impact of categorical perinatal factors on cord blood levels of CC16 was examined with univariate and multivariate regression analyses.

Results In neonates with GA < 32 weeks, cord blood CC16 concentrations were significantly lower compared to neonates with GA between 320/7 and 336/7 weeks (5.4 ± 2.5 compared to 7.6 ± 2.9 ng/mL, p = 0.039). Neonates with prolonged rupture of membranes had significantly lower CC16 compared to those without prolonged rupture of membranes (4.0 ± 1.9 compared to 7.2 ± 2.2, p < 0.001). Finally, neonates with BPD had significantly lower CC16, compared to neonates without BPD (4.2 ± 2.1 compared to 7.0 ± 2.2 ng/mL, p = 0.004).

Prolonged rupture of membranes was significantly negatively associated with CC16 (b = −2.67, 95% confidence interval [CI] −0.49 to −4.85, p = 0.017), after adjusting for GA (b = 0.23, 95% CI 0.03–0.42, p = 0.022), mode of conception, and mode of delivery. Finally, higher CC16 levels were significantly inversely associated with BPD (odds ratio = 0.33, 95% CI 0.12–0.88, p = 0.028), after adjusting for GA (b = 0.27, 95% CI 0.09–0.78, p = 0.015), and birth weight.

Conclusion Prolonged rupture of membranes was significantly negatively associated with cord serum CC16, after adjusting for GA, conception, and delivery mode, and CC16 was significantly inversely associated with BPD, after adjusting for GA and birth weight.

Key Points

  • Neonates with prolonged rupture of membranes had lower CC16 levels.

  • CC16 was significantly negatively associated with BPD.

  • CC16 could be a biomarker of lung injury and BPD.

Keywords

bronchopulmonary dysplasia - infant - mechanical ventilation - respiratory distress syndrome

Authors' Contributions

D.R. and H.C. conceptualized and developed the study design. Data collection was performed by D.R. and A.E.P. A.E.P. performed the ELISA measurements. D.R. performed the statistical analysis and wrote the initial draft. A.E.P. and H.C. critically reviewed and revised the manuscript. All authors approved the final manuscript and agreed to be accountable for all aspects of the work.


Ethical Approval

The study was approved by the institutional review board (Protocol Number 2012P000384/02.10.2012) and exempted from requiring written informed consent. The study was performed in accordance with the Declaration of Helsinki.




Publication History

Received: 11 July 2023

Accepted: 11 December 2023

Article published online:
31 December 2023

© 2023. Thieme. All rights reserved.

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