Decreased Renal and Hepatic Blood Flow with Preeclampsia-Like Histologic Changes was Obtained by Stimulation of the Celiac Ganglion with LPS

ABSTRACT

Decreased renal and hepatic blood flow with preeclampsia-like histologic changes was developed by stimulation of the celiac ganglion with lipopolysaccharide (LPS) in pregnant rabbits. The renal and hepatic blood flow were measured after stimulation of the celiac ganglion with 10 μL (group I), 100 μL (group II) and 300 μL (group III) of LPS (10 mg/mL cone.) and with 100 μL of normal saline (group IV). The bifurcation of the abdominal aorta was also stimulated with 300 μL of LPS (group V). A control experiment was also done in this study (group VI). Histopathological studies of kidney and liver tissue were also performed in this protocol. A significant reduction in renal and hepatic blood flow was observed in pregnant rabbits with the times and dosage dependent on stimulation of the celiac ganglion by LPS. Stimulation of the bifurcation of the abdominal aorta with LPS could not produce any changes in renal and hepatic blood flow. Preeclampsia-like histologic changes of kidney and liver tissue were also observed. These results suggest that exogenous stimulation of the celiac ganglion causes decreased renal and hepatic blood flow, resulting in preeclampsia-like histologic changes of kidney and liver in pregnant rabbits.

Preeclampsia - celiac ganglion - lipopolysaccaride - blood flow - rabbit model