West-Nil-Virus-Infektion bei 12 Pferden in Mitteldeutschland

Zusammenfassung

Gegenstand und Ziel Die Vorstellungsgründe, klinische Symptomatik, Diagnostik, Therapie und Ergebnis der Therapie von 12 Pferden mit klinisch apparenter West-Nil-Virus (WNV)-Infektion werden beschrieben.

Material und Methoden Fallserie

Ergebnisse Die adulten Pferde (Alter 6–18 Jahre, 7 Stuten, 5 Wallache) aus Sachsen oder Sachsen-Anhalt wurden zwischen September 2018 und September 2020 mit unterschiedlichen Vorberichten vorgestellt. Alle Pferde wurden im August oder September vorgestellt und keines der Pferde war gegen das WNV geimpft. Fieber war das häufigste Allgemeinsymptom und trat bei 8/12 Pferden auf. An neurologischen Symptomen traten Muskelfaszikulationen (11/12 Pferde), Ataxie (8/12 Pferde) sowie Hyperästhesie und Kopfschiefhaltung (je 6/12 Pferde) am häufigsten auf. Bei allen Pferden wurde die Infektion mittels Nachweises von IgM sowie neutralisierenden Antikörpern gegen das WNV diagnostiziert, zwei euthanasierte Pferde waren zudem PCR-positiv. Die symptomatische Therapie beinhaltete vor allem nicht-steroidale Antiphlogistika oder Dexamethason sowie Infusionstherapie. Die Dauer des Klinikaufenthaltes betrug im Durchschnitt 7,5 Tage. Sieben Pferde erholten sich laut Besitzerangaben vollständig, für 2 Pferde war keine Information erhältlich.

Schlussfolgerungen und klinische Relevanz Die WNV-Enzephalomyelitis muss in Mitteldeutschland seit 2018 als Differentialdiagnose von im Sommer und Spätsommer auftretenden akuten neurologischen Erkrankungen ungeimpfter Pferdes in Betracht gezogen werden. Die beschriebenen Symptome sowie das Ergebnis der Therapie sind weitgehend deckungsgleich mit Berichten aus Nordamerika und anderen europäischen Ländern.

Abstract

Purpose and aim The presenting complaints, clinical signs, diagnostic evaluation, therapy, and outcome of 12 horses with clinically apparent West-Nile-Virus (WNV) infection are described.

Material and methods Case series

Results The adult horses (age 6–18 years, 7 mares, 5 geldings) from Saxony and Saxony-Anhalt were presented with various clinical histories between September 2018 and September 2020. All horses were presented in August or September and no horse was vaccinated against WNV. Fever as the most common general clinical sign was present in 8/12 horses. The most common neurological signs were muscle fasciculations (11/12 horses), ataxia (8/12 horses), hyperesthesia and head tilt (6/12 horses each). Diagnosis of WNV infection was confirmed by demonstrating IgM antibody and neutralizing antibody production in all horses; 2 euthanized horses also tested positive by PCR. Therapy was symptomatic and primarily included non-steroidal anti-inflammatories or dexamethasone as well as fluid therapy. Duration of hospitalization was 7.5 days on average. According to their owners, seven horses recovered completely, while information was missing for 2 horses.

Conclusions and clinical relevance In eastern-central Germany, WNV-encephalomyelitis must be considered a differential diagnosis for unvaccinated horses with acute neurologic disease occurring in summer and late summer. The reported clinical signs and the outcome of therapy are mostly congruent with reports from North America and other European countries.

Schlüsselwörter

Fallserie - Symptome - Diagnose - Therapie

Key words

Case series - clinical signs - diagnosis - therapy

Publication History

Received: 15 May 2022

Accepted: 10 October 2022

Article published online:
14 December 2022

• Literatur

• 1 Calisher CH, Gould EA. Taxonomy of the virus family Flaviviridae. Adv Virus Res 2003; 59: 1-19
• 2 Bunning ML, Bowen RA, Cropp CB. et al. Experimental infection of horses with West Nile virus. Emerg Infect Dis 2002; 8: 380-386
• 3 Castillo-Olivares J, Wood J. West Nile virus infection of horses. Vet Res 2004; 35: 467-483
• 4 Ostlund EN, Andresen JE, Andresen M. West Nile Encephalitis. Vet Clin North Am Equine Pract 2000; 16: 427-441
• 5 Ward MP, Schuermann JA, Highfield LD. et al. Characteristics of an outbreak of West Nile virus encephalomyelitis in a previously uninfected population of horses. Vet Microbiol 2006; 118: 255-259
• 6 Friedrich-Loeffler-Institut. Hg. Infektion mit dem West-Nil-Virus bei einem Vogel oder Pferd: Amtliche Methode und Falldefinition. In: Amtliche Methodensammlung und Falldefinitionen: Anzeigepflichtige Tierseuchen (2021). OpenAgrar Repository: Friedrich-Loeffler-Inst.; Online unter: Accessed May 08, 2022 at: https://www.openagrar.de/receive/openagrar_mods_00054077
• 7 Beck C, Jimenez-Clavero MA, Leblond A. et al. Flaviviruses in Europe: complex circulation patterns and their consequences for the diagnosis and control of West Nile disease. Int J Environ Res Public Health 2013; 10: 6049-6083
• 8 European Centre for Disease Prevention and Control. West Nile virus infection – Annual Epidemiological Report for 2018 (16.12.2019). Online im Internet: Accessed May 08, 2022 at: https://www.ecdc.europa.eu/en/publications-data/west-nile-virus-infection-annual-epidemiological-report-2018
• 9 Ziegler U, Lühken R, Keller M. et al. West Nile virus epizootic in Germany, 2018. Antiviral Res 2019; 162: 39-43
• 10 Accessed August 25, 2022 at: https://www.ecdc.europa.eu/en/west-nile-fever/surveillance-and-disease-data/historical
• 11 Accessed August 25, 2022 at: https://www.ecdc.europa.eu/en/west-nile-fever/surveillance-and-disease-data/disease-data-ecdc
• 12 Bergmann F, Trachsel DS, Stoeckle SD. et al. Seroepidemiological Survey of West Nile Virus Infections in Horses from Berlin/Brandenburg and North Rhine-Westphalia, Germany. Viruses 2022; 14 (02) 243
• 13 Ganzenberg S, Sieg M, Ziegler U. et al. Seroprevalence and risk factors for equine West Nile Virus infections in eastern Germany, 2020. Viruses 2022; 14: 1191
• 14 Ziegler U, Santos PD, Groschup MH. et al. West Nile virus epidemic in Germany triggered by epizootic emergence, 2019. Viruses 2020; 12: 448
• 15 Leggewie M, Badusche M, Rudolf M. et al. Culex pipiens and Culex torrentium populations from Central Europe are susceptible to West Nile virus infection. One Health 2016; 2: 88-94
• 16 Vogels CB, Göertz GP, Pijlman GP. et al. Vector competence of European mosquitoes for West Nile virus. Emerg Microbes Infect 2017; 6: e96
• 17 Koenraadt CJM, Möhlmann TWR, Verhulst NO. et al. Effect of overwintering on survival and vector competence of the West Nile virus vector Culex pipiens. Parasit Vectors 2019; 12: 147
• 18 https://www.woah.org/en/what-we-do/standards/codes-and-manuals/terrestrial-code-online-access/?id=169&L=1&htmfile=chapitre_wnf.htm
• 19 Weese JS, Baird JD, DeLay J. et al. WNV Encephalomyelitis in horses in Ontario: 28 cases. Can Vet J 2003; 44: 469-473
• 20 Porter MB, Long MT, Getman LM. et al. WNV encephalomyelitis in horses: 46 cases (2001). J Am Vet Med Assoc 2003; 222: 1241-1247
• 21 Salazar P, Traub-Dargatz JL, Morley PS. et al. Outcomes of equids with clinical signs of West Nile virus infection and factors associated with death. J Am Vet Med Assoc 2004; 225: 267-274
• 22 Ostlund EN, Crom RL, Pedersen DD. et al. Equine West Nile encephalitis, United States. Emerg Infect Dis 2001; 7: 665-669
• 23 Ward MP, Levy M, Thacker HL. et al. Investigation of an outbreak of encephalomyelitis caused by West Nile virus in 136 horses. J Am Vet Med Assoc 2004; 225: 84-89
• 24 Davidson AH, Traub-Dargatz JL, Rodeheaver RM. et al. Immunological responses to West Nile virus in vaccinated and clinically affected horses. J Am Vet Med Assoc 2005; 226: 240-245
• 25 Tanner JM, Traub-Dargatz JL, Hill AE. et al. Evaluation of factors associated with positive IgM capture ELISA results in equids with clinical signs compatible with West Nile virus infection: 1,017 cases (2003). J Am Vet Med Assoc 2006; 228: 414-421
• 26 Weiss D, Carr D, Kellachan J. et al. Clinical findings of WNV infection in hospitalized patients. New York and New Jersey, 2000. Emerg Infect Dis 2001; 7: 654-658
• 27 Snook CS, Hyman SS, Del Piero F. et al. WNV encephalomyelitis in eight horses. J Am Vet Med Assoc 2001; 218: 1576-1579
• 28 Friedrich-Loeffler-Institut. TSIS – Tierseucheninformationssystem. Online im Internet: Accessed May 08, 2022 at: https://tsis.fli.de/Reports/Info_SO.aspx?ts=416&guid=0068912b-5720–4316-b1d7–75e61608a2e3
• 29 Porter RS, Leblond A, Lecollinet S. et al. Clinical Diagnosis of West Nile Fever in equids by classification and regression tree (CART) analysis and comparative study of clinical appearance in three European countries. Transbound Emerg Dis 2011; 58: 197-205
• 30 Epp T, Waldner C, Townsend HGG. A case-control study of factors associated with development of clinical disease due to West Nile virus, Saskatchewan 2003. .  Eq Vet J 2007; 39: 498-503
• 31 Garcia I Bocanegra, Belkhiria J, Napp D. et al. Epidemiology and spatio-temporal analysis of West Nile virus in horses in Spain between 2010 and 2016. Transbound Emerg Dis 2018; 65: 567-577
• 32 Cantile C, Di Guardo G, Eleni C. et al. Clinical and neuropathological features of West Nile virus equine encephalomyelitis in Italy. Eq Vet J 2000; 32: 31-35
• 33 de Heus P, Kolodziejek J, Camp JV. et al. Emergence of West Nile virus lineage 2 in Europe: Characteristics of the first seven cases of West Nile neuroinvasive disease in horses in Austria. Transbound Emerg Dis 2020; 67: 1189-1197
• 34 Cantile C, Del Piero F, Di Guardo G. et al. Pathologic and immunohistochemical findings in naturally occurring West Nile Virus infection in horses. Vet Pathol 2001; 38: 414-421
• 35 Rech R, Barros C. Neurologic Disease in Horses. Vet Clin North Am Equine Pract 2015; 31: 281-306
• 36 Toplu N, Oguzoglu TC, Ural K. et al. West Nile Virus infection in horses : detection by immunohistochemistry, in situ hybridization, and ELISA. Vet Pathol 2015; 52: 1073-1076
• 37 Cantile C, Youssef S. Flaviviral encephalitides. In: Maxie MG. Hg. Jubb, Kennedy, and Palmer’s pathology of domestic animals. 6. Aufl.. Elsevier; 2015: 373-375
• 38 Durand B, Chevalier V, Pouillot R. et al. West Nile virus outbreak in horses, Southern France, 2000: results of a serosurvey. Emerg Infect Dis 2002; 8: 777-782
• 39 Steinman A, Banet C, Sutton GA. et al. Clinical signs of West Nile virus encephalomyelitis in horses during the outbreak in Israel in 2000. Vet Rec 2002; 151: 47-49
• 40 Delcambre GH, Long MT. Flavivirus encephalitides. In Sellon DC, Long M. Hg. Equine infectious diseases. 2. Aufl.. St. Louis: Saunders; 2014: 217-226
• 41 Long M, Porter MB. et al. Preliminary data regarding the subclinical exposure rate of horses to West Nile Virus during the 2001 Florida enzootic. In: Proceedings. 49th Annu Meet Am Assoc Equine Prat. 2003: 387-398
• 42 Khatibzadeh SM, Gold CB, Keggan AE. et al. West Nile virus-specific immunoglobulin isotype responses in vaccinated and infected horses. Am J Vet Res 2015; 76: 92-100
• 43 Stasiak K, Dunowska M, Rola J. Prevalence and sequence analysis of equid herpesviruses from the respiratory tract of Polish horses. Virol J 2018; 15: 106
• 44 Wamsley HL, Alleman AR, Porter MB. et al. Findings in cerebrospinal fluids of horses infected with West Nile virus: 30 cases (2001). J Am Vet Med Assoc 2002; 221: 1303-1305
• 45 Paré J, Moore A. West Nile virus in horses – What do you need to know to diagnose the disease?. Can Vet J 2018; 59: 1119-1120
• 46 Read AJ, Finlaison DS, Gu X. et al. Clinical and epidemiological features of West Nile virus equine encephalitis in New South Wales, Australia, 2011. Austr Vet J 2019; 97 (05) 133-143
• 47 McIver K. Recovery from a severe West Nile Virus infection in a one-year-old stallion following interferon alpha-2b treatment: an equine model for human therapy. Master of Public Health Report. 2021 https://krex.k-state.edu/dspace/handle/2097/41528
• 48 MacKay RJ. West Nile Encephalitis. In: Robinson NE, Sprayberry KA. Hg. Current Therapy in Equine Medicine. 6. Aufl.. St.Louis: Saunders; 2009: 622-625
• 49 Sinigaglia A, Peta E, Riccetti S, Barzon L. New avenues for therapeutic discovery against West Nile virus. Expert Opin Drug Dis. 2020