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J Reconstr Microsurg 2009; 25(5): 283-288
DOI: 10.1055/s-0029-1202552
© Thieme Medical Publishers

Effect of IIb-IIIa Glycoprotein Inhibitors in a Partial Limb Amputation Model Submitted to Warm Ischemia in Rats

Marcelo Sacramento Cunha1 , José Carlos Faes da Silva2 , Hugo Alberto Nakamoto2 , Fillipe Almeida Machado1 , Marcus Castro Ferreira2
  • 1Division of Plastic Surgery, Hospital of Clinics, Faculty of Medicine, Federal University of Bahia–UFBA, Bahia, Brazil
  • 2Division of Plastic Surgery, Hospital of Clinics, Faculty of Medicine, University of São Paulo–USP, São Paulo, Brazil
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Publication History

Publication Date:
12 March 2009 (online)

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ABSTRACT

Viability and functional results of a segment replantation depend on the prevention of deleterious effects of ischemia. Prolonged ischemia leads to alterations in the microcirculation: thrombosis, edema, production of oxygen free radicals, and platelet aggregation. The effect of IIb-IIIa glycoprotein inhibitors was tested in a partial limb amputation model submitted to warm ischemia. The male Wistar rats were divided into four groups: G1 with 0 hours of ischemia and saline (n = 20), G2 with 6 hours of ischemia and saline (n = 24), G3 with 6 hours of ischemia and abciximab (n = 23), and G4 with 6 hours of ischemia and tirofiban (n = 29). The limbs were observed for 7 days and classified as viable or nonviable. Viability and mortality rates were obtained and analyzed by Q-square and Fisher exact tests (p < 0.05). The viability rates were 100% (G1), 30% (G2), 77.78% (G3), and 80.95% (G4). G2 was statistically different from G1, G3, and G4. G1, G3, and G4 were not statistically different. Transoperative and postoperative mortalities were not statistically different. The administration of abciximab and tirofiban improved limb salvage after ischemia and reperfusion and did not modify mortality rates significantly.

KEYWORDS

No-reflow phenomenon - platelet - rat limb

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Marcelo Sacramento CunhaM.D. 

Division of Plastic Surgery, Hospital of Clinics, Faculty of Medicine, Federal University of Bahia–UFBA, Rua João das Botas

No. 89/601 Canela, Salvador, Bahia, Brazil, 40110-160

Email: cunha.ms@pop.com.br

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