Arzneimittelforschung 2008; 58(9): 429-434
DOI: 10.1055/s-0031-1296534
Cardiac Drugs · Cardiac Stimulants · Coronary Drugs
Editio Cantor Verlag Aulendorf (Germany)

Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Trimetazidine in Human Plasma

Jianjun Zou
1   Department of Clinical Pharmacology, anjing First Hospital Affiliated to Nanjing Medical University, Nanjing, The People’s Republic of China
,
Li Dai
2   Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, The People’s Republic of China
,
Guang Ji Wang
3   Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, The People’s Republic of China
,
Yubing Zhu
1   Department of Clinical Pharmacology, anjing First Hospital Affiliated to Nanjing Medical University, Nanjing, The People’s Republic of China
,
Hongwei Fan
1   Department of Clinical Pharmacology, anjing First Hospital Affiliated to Nanjing Medical University, Nanjing, The People’s Republic of China
,
Da Wei Xiao
1   Department of Clinical Pharmacology, anjing First Hospital Affiliated to Nanjing Medical University, Nanjing, The People’s Republic of China
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Publikationsdatum:
19. Dezember 2011 (online)

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Abstract

A sensitive liquid chromatography-tandem mass spectrometry (LC/MS/MS) method was developed and validated for the determination of trimetazidine (CAS 13171-25-0) in human plasma, using pseudoephedrine as internal standard (IS). Plasma samples were simply pretreated with methanol for deproteinization. The chromatographic separation was performed on a C18 column with a mobile phase of 3 mmol/L ammonium acetate solution-methanol (15:85, v/v) at a flow rate of 0.3 mL/min. The chromatographic separation was achieved in less than 3.2 min. The linearity was established over the concentration range of 1–100 ng/mL. Both intra- and inter-batch standard deviations were less than 9.5%. The method was successfully applied to study the relative bioavailability of trimetazidine hydrochloride tablets in healthy Chinese volunteers and the pharmacokinetic parameters of the reference and test tablets were compared.