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Int J Sports Med 2014; 35(10): 822-827
DOI: 10.1055/s-0033-1363982
Physiology & Biochemistry
© Georg Thieme Verlag KG Stuttgart · New York

Oxidative Stress and Inflammation Response Following Aerobic Exercise: Role of Ethnicity

M. J. McKenzie
1   Winston Salem State University, Human Performance and Sport Sciences, Winston-Salem, United States
,
A. Goldfarb
2   UNC Greensboro, Kinesiology, Greensboro, United States
,
R. S. Garten
3   George E. Whalen Veterans Affairs Medical Center, Aging, Salt Lake City, United States
,
L. Vervaecke
2   UNC Greensboro, Kinesiology, Greensboro, United States
› Author Affiliations
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Publication History



accepted after revision 27 November 2013

Publication Date:
15 April 2014 (online)

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Abstract

African-Americans are at a significantly greater risk for developing several diseases and conditions. These conditions often have underlying oxidative stress mechanisms. Therefore the purpose of this investigation was to ascertain the post-exercise oxidative response to a single bout of aerobic exercise in African-American and Caucasian college-age females. A total of 10 African-American and 10 Caucasian females completed the study. Each subject had her VO2 max measured while exercising on a treadmill. A week later, each subject returned to the laboratory and performed a 30-min run at 70% of her VO2max. Blood samples were taken immediately prior to and following exercise for analysis. Lipid hydroperoxides, protein carbonyls, malondialdehyde, xanthine oxidase, glutathione in the reduced (GSH) and oxidized (GSSG) forms, TNFα and interleukin 6 were measured from blood taken before and after exercise. Significance was set at p≤0.05 a priori. Xanthine oxidase was the only measure that did not significantly increase following exercise. All other markers showed a significant elevation in response to the exercise bout with no difference between groups except that the Caucasian group had significantly higher malondialdehyde post-exercise compared to the African-American group. This cohort of college-age African-American and Caucasian females showed little difference in their response to a single 30-min run at 70% of their max in the markers of oxidative stress within the blood.

Key words

glutathione - lipid hydroperoxides - malondialdehyde - protein carbonyl - xanthine oxidase - interleukin 6
 

  • References

  • 1 Abbey EL, Rankin JW. Effect of quercetin supplementation on repeated-sprint performance, xanthine oxidase activity, and inflammation. Int J Sport Nutr Exerc Metab 2011; 21: 91-96
    PubMedGoogle Scholar
  • 2 Alessio HM, Goldfarb AH, Cao G. Exercise-induced oxidative stress before and after vitamin C supplementation. Int J Sport Nutr 1997; 7: 1-9
    PubMedGoogle Scholar
  • 3 Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, Wiley RL. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc 2000; 32: 1576-1581
    PubMedGoogle Scholar
  • 4 Bloomer RJ, Goldfarb AH. Anaerobic exercise and oxidative stress: a review. Can J Appl Physiol 2004; 29: 245-263
    CrossrefPubMedGoogle Scholar
  • 5 Bloomer RJ, Goldfarb AH, McKenzie MJ. Oxidative stress response to aerobic exercise: Comparison of antioxidant supplements. Med Sci Sport Exer 2006; 38: 1098-1105
    CrossrefPubMedGoogle Scholar
  • 6 Bloomer RJ, Goldfarb AH, Wideman L, McKenzie MJ, Consitt LA. Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J Strength and Cond Res 2005; 19: 1-10
    PubMedGoogle Scholar
  • 7 Brown MD, Feairheller DL, Thakkar S, Veerabhadrappa P, Park JY. Racial differences in tumor necrosis factor-alpha-induced endothelial microparticles and interleukin-6 production. Vasc Health Risk Manag 2011; 7: 541-550
    PubMedGoogle Scholar
  • 8 Christians ES, Benjamin IJ. Proteostasis and Redox state in the heart. Am J Physiol 2011; 302: H24-H37
    PubMedGoogle Scholar
  • 9 Christiansen T, Bruun JM, Paulsen SK, Olholm J, Overgaard K, Pedersen SB, Richelsen B. Acute exercise increases circulating inflammatory markers in overweight and obese compared with lean subjects. Eur J Appl Physiol 2013; 113: 1635-1642
    CrossrefPubMedGoogle Scholar
  • 10 Ding J, Diez Roux AV, Nieto FJ, Diez Roux AV, McNamara RL, Hetmanski JB, Taylor Jr HA, Tyroler HA. Racial disparity in long-term mortality rate after hospitalization for myocardial infarction: the Atherosclerosis Risk in Communities study. Am Heart J 2003; 146: 459-464
    CrossrefPubMedGoogle Scholar
  • 11 Doumatey AP, Chen G, Tekola Ayele F, Zhou J, Erdos M, Skinner D, Huang H, Adeleye J, Balogun W, Fasanmade O, Johnson T, Oli J, Okafor G, Amoah A, Eghan BA, Agenium-Boateng K, Acheampong J, Adebamowo C, Gerry NP, Christman MF, Adeyemo A, Rotimi CN. C-reactive protein (CRP) promoter polymorphisms influence circulating CRP levels in a genome-wide association study of African-Americans. Hum Mol Genet 2012; 21: 3063-3072
    CrossrefPubMedGoogle Scholar
  • 12 Feairheller DL, Park JY, Rizzo V, Kim B, Brown MD. Racial differences in the responses to shear stress in human umbilical vein endothelial cells. Vasc Health Risk Manag 2011; 7: 425-431
    PubMedGoogle Scholar
  • 13 Febbraio MA, Pedersen BK. Contraction-induced myokine production and release: is skeletal muscle an endocrine organ?. Exerc Sport Sci Rev 2005; 33: 114-119
    CrossrefPubMedGoogle Scholar
  • 14 Fisher G, Hyatt TC, Hunter GR, Oster RA, Desmond RA, Gower BA. Markers of inflammation and fat distribution following weight loss in African-American and white women. Obesity (Silver Spring) 2012; 20: 715-720
    CrossrefPubMedGoogle Scholar
  • 15 Goldfarb AH. Antioxidants: role of supplementation to prevent exercise-induced oxidative stress. Med Sci Sports Exerc 1993; 25: 232-236
    PubMedGoogle Scholar
  • 16 Goldfarb AH, Bloomer RJ, McKenzie MJ. Effect of Microhydrin on blood lactate, Protein Carbonyls, and Glutathione staus in Rats Before and After Aerobic Exercise. Int J Sport Nutr Exerc Metab 2004; 14: 550-559
    PubMedGoogle Scholar
  • 17 Goldfarb AH, Bloomer RJ, McKenzie MJ. Combined antioxidant treatment effects on blood oxidative stress to eccentric exercise. Med Sci Sport and Exer 2005; 37: 234-239
    PubMedGoogle Scholar
  • 18 Goldfarb AH, Bloomer RJ, McKenzie MJ. Effect of combination antioxidant treatment on biomarkers of oxidative stress following eccentric exercise. Int J Sport Nutr Exerc Metab 2004; 14: 550-559
    PubMedGoogle Scholar
  • 19 Goldfarb AH, You T, Bloomer RJ, Landes S, Murphy C. Blood oxidative stress markers in response to aerobic exercise. Med Sci Sport Exec 2002; 34: S249
    PubMedGoogle Scholar
  • 20 Gore M, Fiebig R, Hollander J, Leeuwenburgh C, Ohno H, Ji LL. Endurance training alters antioxidant enzyme gene expression in rat skeletal muscle. Can J Physiol Pharmacol 1998; 76: 1139-1145
    CrossrefPubMedGoogle Scholar
  • 21 Harriss DJ, Atkinson G. Update – Ethical standards in sport and exercise science research. Int J Sports Med 2013; 34: 1025-1028
    Article in Thieme ConnectPubMedGoogle Scholar
  • 22 Harzand A, Tamariz L, Hare JM. Uric acid, heart failure survival, and the impact of xanthine oxidase inhibition. Congest Heart Fail 2012; 18: 179-182
    CrossrefPubMedGoogle Scholar
  • 23 Hozawa A, Folsom AR, Sharrett AR, Chambless LE. Absolute and attributable risks of cardiovascular disease incidence in relation to optimal and borderline risk factors: comparison of African-American with white subjects – Atherosclerosis Risk in Communities Study. Arch Intern Med 2007; 167: 573-579
    CrossrefPubMedGoogle Scholar
  • 24 Huang CC, Lin TJ, Lu YF, Chen CC, Huang CY, Lin WT. Protective effects of L-arginine supplementation against exhaustive exercise-induced oxidative stress in young rat tissues. Chin J Physiol 2009; 52: 306-315
    CrossrefPubMedGoogle Scholar
  • 25 Jenkins R, Goldfarb AH. Introduction: oxidant stress, aging and exercise. Med Sci Sports Exerc 1993; 25: 210-212
    PubMedGoogle Scholar
  • 26 Ji LL. Exercise-induced modulation of antioxidant defense. Ann N Y Acad Sci 2002; 959: 82-92
    CrossrefPubMedGoogle Scholar
  • 27 Jovov B, Araujo-Perez F, Sigel CS, Stratford JK, McCoy AN, Yeh JJ, Keku T. Differential gene expression between African-American and European American colorectal cancer patients. PLoS One 2012; 7: e30168
    CrossrefPubMedGoogle Scholar
  • 28 Lloyd-Jones D, Adams RJ, Brown TM, Carnethon M, Dai S, De Simone G, Ferguson TB, Ford E, Furie K, Gillespie C, Go A, Greenlund K, Haase N, Hailpern S, Ho PM, Howard V, Kissela B, Kittner S, Lacklan D, Lisabeth L, Marelli A, McDermott MM, Meigs J, Mozaffarian D, Mussolino M, Nichol G, Roger VL, Rosamond W, Sacco R, Sorlie P, Stafford R, Thom T, Wasserthiel-Smoller S, Wong ND, Wylie-Rosett J. Executive summary: heart disease and stroke statistics – 2010 update: a report from the American Heart Association. Circulation 2010; 121: 948-954
    CrossrefPubMedGoogle Scholar
  • 29 Lloyd-Jones D, Adams RJ, Brown TM, Carnethon M, Dai S, De Simone G, Ferguson TB, Ford E, Furie K, Gillespie C, Go A, Greenlund K, Haase N, Hailpern S, Ho PM, Howard V, Kissela B, Kittner S, Lacklan D, Lisabeth L, Marelli A, McDermott MM, Meigs J, Mozaffarian D, Mussolino M, Nichol G, Roger VL, Rosamond W, Sacco R, Sorlie P, Stafford R, Thom T, Wasserthiel-Smoller S, Wong ND, Wylie-Rosett J. Heart disease and stroke statistics – 2010 update: a report from the American Heart Association. Circulation 2010; 121: e46-e215
    CrossrefPubMedGoogle Scholar
  • 30 Lloyd-Jones DM. Cardiovascular risk prediction: basic concepts, current status, and future directions. Circulation 2010; 121: 1768-1777
    CrossrefPubMedGoogle Scholar
  • 31 Moorman JE, Rudd RA, Johnson CA, King M, Minor P, Bailey C, Scalia MR, Akinbami LJ. National surveillance for asthma – United States, 1980-2004. Morbidity and mortality weekly report Surveillance summaries 2007; 56: 1-54
    PubMedGoogle Scholar
  • 32 Nieman DC, Davis JM, Henson DA, Walberg-Rankin J, Shute M, Dumke CL, Utter AC, Vinci DM, Carson JA, Brown A, Lee WJ, McAnulty SR, McAnulty LS. Carbohydrate ingestion influences skeletal muscle cytokine mRNA and plasma cytokine levels after a 3-h run. J Appl Physiol 2003; 94: 1917-1925
    CrossrefPubMedGoogle Scholar
  • 33 Pattwell DM, McArdle A, Morgan JE, Morgan JE, Patridge TA, Jackson MJ. Release of reactive oxygen and nitrogen species from contracting skeletal muscle cells. Free Radic Biol Med 2004; 37: 1064-1072
    CrossrefPubMedGoogle Scholar
  • 34 Pedersen BK. The diseasome of physical inactivity – and the role of myokines in muscle – fat cross talk. J Physiol 2009; 587: 5559-5568
    CrossrefPubMedGoogle Scholar
  • 35 Petersen AM, Pedersen BK. The anti-inflammatory effect of exercise. J Appl Physiol 2005; 98: 1154-1162
    CrossrefPubMedGoogle Scholar
  • 36 Powers SK, Jackson MJ. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiol Rev 2008; 88: 1243-1276
    CrossrefPubMedGoogle Scholar
  • 37 Powers SK, Ji LL, Leeuwenburgh C. Exercise training-induced alterations in skeletal muscle antioxidant capacity: a brief review. Med Sci Sports Exerc 1999; 31: 987-997
    CrossrefPubMedGoogle Scholar
  • 38 Scott JP, Sale C, Greeves JP, Casey A, Dutton J, Fraser WD. Cytokine response to acute running in recreationally-active and endurance-trained men. Eur J Appl Physiol 2013; 113: 1871-1882
    CrossrefPubMedGoogle Scholar
  • 39 Sen CK, Packer L, Hanninen O. Handbook of oxidants and antioxidants in exercise. Amsterdam; Oxford: Elsevier; 2000
    Google Scholar
  • 40 Sturgeon KM, Feairheller DL, Diaz KM, Williamson ST, Veerabhadrappa P, Brown MD. Clinical risk factors demonstrate an age-dependent relationship with oxidative stress biomarkers in African-Americans. Ethn Dis 2010; 20: 403-408
    PubMedGoogle Scholar
  • 41 Teke T, Maden E, Kiyici A, Korkmaz C, Gok M, Ozer F, Imecik O, Uzun K. Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats. Exp Ther Med 2012; 4: 121-124
    PubMedGoogle Scholar
  • 42 Textor SC. Renovascular hypertension in 2007: where are we now?. Curr Cardiol Rep 2007; 9: 453-461
    CrossrefPubMedGoogle Scholar
  • 43 Whaley-Connell A, McCullough PA, Sowers JR. The role of oxidative stress in the metabolic syndrome. Rev Cardiovasc Med 2011; 12: 21-29
    PubMedGoogle Scholar