Hepatoprotective Effect of Matricaria chamomilla.L in Paraquat Induced Rat Liver Injury

Abstract

Paraquat (PQ), an effective and widely used herbicide, has been proven to be safe when appropriately applied to eliminate weeds. However, PQ poisoning is an extremely frustrating clinical condition with a high mortality and with a lack of effective treatments in humans. PQ is known to induce injury via a redox cyclic reaction. The purpose of this study was to investigate the effect of aqueous extract Matricaria chamomilla.L (M. chamomilla) against PQ-induced liver injury in association with its antioxidant activity.

The male rats were treated by gastric gavage daily with PQ (5 mg/kg/day) and M. chamomilla (50 mg/kg/day) were administered alone or in combination for 7 days. After treatments, total antioxidant capacity (TAC), total thiol molecules (TTG) levels and catalase (CAT) activity in liver tissue were measured. At the end of the experiment, plasma and lung tissue of the animals was separated. The activity of enzymatic scavengers such as CAT, TAC and TTG were measured in liver homogenate.

In this sample, the TAC and TTG were lower in the PQ group as compared with control group. Co-administration of PQ with M. chamomilla extract increased TAC and TTG in liver tissue as compared with PQ group.

In conclusion, M. chamomilla as natural antioxidant may be considered beneficial for the protection oxidative liver injury in PQ poisoning.

Key words

paraquat - matricaria chamomilla.L - oxidative stress - liver - rat

• References

• 1 Bismuth C, Garnier R, Baud F et al. Paraquat poisoning. Drug Saf 1990; 5: 243-251
• 2 Bus JS, Cagen SZ, Olgaard M et al. A mechanism of paraquat toxicity in mice and rats. Toxicology and applied pharmacology 1976; 35: 501-513
• 3 Smith L. Paraquat toxicity. Philosophical Transactions of the Royal Society of London B, Biological Sciences 1985; 311: 647-657
• 4 Smith L. Mechanism of paraquat toxicity in lung and its relevance to treatment. Human & Experimental Toxicology 1987; 691: 31-36
• 5 Papiris S, Maniati M, Kyriakidis V et al. Pulmonary damage due to paraquat poisoning through skin absorption. Respiration 2009; 62: 101-103
• 6 Kim S-j, Gil H-W, Yang J-O et al. The clinical features of acute kidney injury in patients with acute paraquat intoxication. Nephrology Dialysis Transplantation 2009; 24: 1226-1232
• 7 Lock EA, Ishmael J. The acute toxic effects of paraquat and diquat on the rat kidney. Toxicology and applied pharmacology 1979; 50: 67-76
• 8 Amirshahrokhi K, Bohlooli S. Effect of Methylsulfonylmethane on Paraquat-Induced Acute Lung and Liver Injury in Mice. Inflammation 2013; 1-11
• 9 Hirai K-I, Ikeda K, Wang G-Y. Paraquat damage of rat liver mitochondria by superoxide production depends on extramitochondrial NADH. Toxicology 1992; 72: 1-16
• 10 Pasi A. The toxicology of paraquat, diquat and morfamquat: H. Hubert Publishers; 1978
• 11 McCormack AL, Atienza JG, Johnston LC et al. Role of oxidative stress in paraquat-induced dopaminergic cell degeneration. Journal of neurochemistry 2005; 93: 1030-1037
• 12 Suntres ZE. Role of antioxidants in paraquat toxicity. Toxicology 2002; 180: 65-77
• 13 Dodge A. The mode of action of the bipyridylium herbicides, paraquat and diquat. Endeavour 1971; 30: 130-135
• 14 Bateman D. Pharmacological treatments of paraquat poisoning. Human toxicology 1987; 6: 57
• 15 Vale J, Meredith T, Buckley B. Paraquat poisoning: clinical features and immediate general management. Human & Experimental Toxicology 1987; 6: 41-47
• 16 Avallone R, Zanoli P, Puia G et al. Pharmacological profile of apigenin, a flavonoid isolated from Matricaria chamomilla. Biochemical pharmacology 2000; 59: 1387
• 17 Owlia P, Rasooli I, Saderi H. Antistreptococcal and antioxidant activity of essential oil from Matricaria chamomilla L. Res J Biol Sci 2007; 2: 237-239
• 18 Cemek M, Kağa S, Şimşek N et al. Antihyperglycemic and antioxidative potential of Matricaria chamomilla L. in streptozotocin-induced diabetic rats. Journal of natural medicines 2008; 62: 284-293
• 19 Salama R. Matricaria chamomilla attenuates cisplatin nephrotoxicity. Saudi Journal of Kidney Diseases and Transplantation 2012; 23: 765
• 20 Salama RHM, Abd-El-Hameed NA-M, Abd-El-Ghaffar SK et al. Nephroprotective Effect of Nigella sativa and Matricaria chamomilla in Cisplatin Induced Renal Injury. 2011
• 21 Ghazi-Khansari M, Mohammadi-Bardbori A. Captopril ameliorates toxicity induced by paraquat in mitochondria isolated from the rat liver. Toxicology in vitro 2007; 21: 403-407
• 22 Macchioni F, Perrucci S, Cecchi F et al. Acaricidal activity of aqueous extracts of camomile flowers, Matricaria chamomilla, against the mite Psoroptes cuniculi. Medical and Veterinary Entomology 2004; 18: 205-207
• 23 Johansson LH, Håkan Borg L. A spectrophotometric method for determination of catalase activity in small tissue samples. Analytical biochemistry 1988; 174: 331-336
• 24 Benzie IF, Strain J. [2] Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in enzymology 1999; 299: 15-27
• 25 Hu M, Dillard C. Plasma SH and GSH measurement. Methods Enzymol 1994; 233: 87
• 26 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 1976; 72: 248-254
• 27 Singh N, Kamath V, Narasimhamurthy K et al. Protective effect of potato peel extract against carbon tetrachloride-induced liver injury in rats. Environmental toxicology and pharmacology 2008; 26: 241-246
• 28 Babenko NA, Shakhova EG. Effects of Chamomilla recutita flavonoids on age-related liver sphingolipid turnover in rats. Experimental gerontology 2006; 41: 32-39
• 29 Sies H. Oxidative stress: oxidants and antioxidants. Experimental physiology 1997; 82: 291-295
• 30 Limón-Pacheco J, Gonsebatt ME. The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2009; 674: 137-147
• 31 Yousef MI, Omar SA, El-Guendi MI et al. Potential protective effects of quercetin and curcumin on paracetamol-induced histological changes, oxidative stress, impaired liver and kidney functions and haematotoxicity in rat. Food and Chemical Toxicology 2010; 48: 3246-3261
• 32 Jurczuk M, Brzoska M, Moniuszko-Jakoniuk J et al. Antioxidant enzymes activity and lipid peroxidation in liver and kidney of rats exposed to cadmium and ethanol. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 2004; 42: 429
• 33 Sittipunt C. Paraquat poisoning. Respir Care 2005; 50: 383-385
• 34 Morán JM, Ortiz-Ortiz MA, Ruiz-Mesa LM. Nitric oxide in paraquat-mediated toxicity: A review. Journal of biochemical and molecular toxicology 2010; 24: 402-409
• 35 Ranjbar A, Pasalar P, Sedighi A et al. Induction of oxidative stress in paraquat formulating workers. Toxicology letters 2002; 131: 191-194
• 36 Melchiorri D, Reiter RJ, Sewerynek E et al. Paraquat toxicity and oxidative damage: Reduction by melatonin. Biochemical pharmacology 1996; 51: 1095-1099
• 37 Block E. Potentiation of acute paraquat toxicity by vitamin E deficiency. Lung 1979; 156: 195-203
• 38 Dawson JR, Norbeck K, Anundi I et al. The effectiveness of N-acetylcysteine in isolated hepatocytes, against the toxicity of paracetamol, acrolein, and paraquat. Archives of toxicology 1984; 55: 11-15
• 39 Djeridane A, Yousfi M, Nadjemi B et al. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry 2006; 97: 654-660
• 40 Ahmed RS, Suke SG, Seth V et al. Protective effects of dietary ginger (Zingiber officinales Rosc.) on lindane-induced oxidative stress in rats. Phytotherapy Research 2008; 22: 902-906
• 41 Abdoul-Latif FM, Nabil M, Edou P et al. Antimicrobial and antioxidant activities of essential oil and methanol extract of Matricaria chamomilla L. from Djibouti. Journal of Medicinal Plants Research 2011; 5: 1512-1517
• 42 Garetz SL, Rhee DJ, Schacht J. Sulfhydryl compounds and antioxidants inhibit cytotoxicity to outer hair cells of a gentamicin metabolite in vitro. Hearing research 1994; 77: 75-80
• 43 Deneke SM. Thiol-based antioxidants. Current topics in cellular regulation 2001; 36: 151-180
• 44 Cemek M, Yilmaz E, Büyükokuroglu ME. Protective effect of Matricaria chamomilla on ethanol-induced acute gastric mucosal injury in rats. Pharmaceutical Biology 2010; 48: 757-763
• 45 Gomaa A, Hashem T, Mohamed M et al. Matricaria chamomilla extract inhibits both development of morphine dependence and expression of abstinence syndrome in rats. Journal of pharmacological sciences 2003; 92: 50-55
• 46 Kováčik J, Bačkor M. Oxidative status of Matricaria chamomilla plants related to cadmium and copper uptake. Ecotoxicology 2008; 17: 471-479
• 47 Kumar A, Ahmad I, Shukla S et al. Effect of zinc and paraquat co-exposure on neurodegeneration: Modulation of oxidative stress and expression of metallothioneins, toxicant responsive and transporter genes in rats. Free Radic Res 2010; 10: 3109-3113
• 48 Fei Q, Douglas W. Maneb potentiates paraquat neurotoxicity by inducing key Bcl-2 family members. J Neurochem 2008; 105: 2091-2097