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Drug Res (Stuttg) 2015; 65(07): 347-353
DOI: 10.1055/s-0034-1382064
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Stigmasterol Tetracosanoate, a New Stigmasterol Ester from the Egyptian Blepharis ciliaris

M. A. El-Shanawany
1   Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
,
H. M. Sayed
1   Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
,
S. R. M. Ibrahim
1   Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
,
M. A. A. Fayed
1   Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt
› Author Affiliations
Further Information

Publication History

received 20 May 2014

accepted 01 June 2014

Publication Date:
03 July 2014 (online)

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Abstract

A new stigmasterol ester: stigmasterol tetracosanoate (3), along with 7 compounds: β-sitosterol (1), stigmasterol (2), (2S,3S,4R)-2[(2′R)-2′-(hydroxyeicosanoyl amino) octadecane-1,3,4-triol (4), apigenin (5), β-sitosterol-3-O-β-D-glucopyranoside (6), stigmasterol-3-O-β-D-glucopyranose (7), and apigenin-7-O-β-D-glucopyranoside (8) were isolated from Blepharis ciliaris aerial parts. Compounds 1, 2, and 5–7 are reported here for the first time from the plant and 4 for the first time from the family. GCMS analysis revealed the presence of 45 fatty acids, 53 hydrocarbons, and 24 sterols. The different fractions exhibited mild cytotoxic in brine shrimp assay and anti-hyperglycaemic activities. The EtOAc fraction and TME (total MeOH extract) showed weak anti-malarial activity against P. falciparum. The CHCl3 fraction gave potent ­anti-inflammatory activity compared with indomethacin.

Key words

Blepharis ciliaris - stigmasterol tetracosanoate - GCMS - cytotoxic - anti-malarial - anti-inflammatory - anti-hyperglycaemic
 

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