Topical anti-inflammatory activity of the hydroethanolic extract from Kalanchoe brasiliensis Cambess. (Crassulaceae) leaves

O Mayorga; J Florencio; B Santos; O do Amaral Corrêa José; O Sousa; S Alves Maria

doi:10.1055/s-0037-1608178

Planta Medica International Open, Table of Contents

Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608178

Poster Session

Georg Thieme Verlag KG Stuttgart · New York

Topical anti-inflammatory activity of the hydroethanolic extract from Kalanchoe brasiliensis Cambess. (Crassulaceae) leaves

Authors

O Mayorga

¹Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
J Florencio

¹Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
B Santos

¹Graduate Program in Pharmaceutical Sciences, Federal University of Juiz de Fora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
O do Amaral Corrêa José

²Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
O Sousa

²Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil
S Alves Maria

²Department of Pharmaceutical Sciences, Faculty of Pharmacy, Federal University of Juiz de Fora, Rua José Lourenço Kelmer s/n, Campus Universitário, Bairro São Pedro, CEP 36036 – 900, Juiz de Fora, Brazil

Abstract

Full Text

Kalanchoe brasiliensis Cambess. (Crassulaceae), known as “saião”, is a plant used in folk medicine for the treatment of inflammation, injuries, abscesses, and enlarged ganglia.^1,2 The present study evaluated the topical anti-inflammatory effect of the hydroethanolic extracts EHJ50 (50%) and EHJ70 (70%) obtained from K. brasiliensis leaves. Using doses of 0.10, 0.50 and 1.0 mg/ear of the extracts, the anti-inflammatory activity was assessed by Croton oil-induced acute ear edema method³, determination of inflammatory enzymes (MPO and NAG) and proinflammatory mediators (NO, IL-6 and TNF-α) and histopathological analysis. After 24h of treatment with EHJ50 and EHJ70, the inhibition of ear edema ranged from 62.03 to 80.34%. These extracts also reduced MPO (55.27 to 81.74%) and NAG (69.04 to 80.82%), while the NO levels decreased from 46.74 to 67.43%. The dose of 1.0 mg/ear was able to inhibit IL-6 (EHJ50 = 20.59% and EHJ70 = 22.78%) and TNF-α (EHJ50 = 16.71% and EHJ70 = 23.71%). These results suggest that K. brasiliensis has topical anti-inflammatory effect and can be a promising source for the development of phytotherapics for skin disorders.

This investigation was supported by UFJF, FAPEMIG, CAPES, OEA and GCUB.

[1] Costa SS, Jossang A, Bodo B, Souza ML, Moraes VL. J Nat Prod 1994; 57: 1503 – 1510.

[2] Mourão RH, Santos FO, Franzotti EM, Moreno MP, Antoniolli AR. Phytother Res 1999; 13: 352 – 354.

[3] Schiantarelli P, Cadel S, Acerbi D, Pavesi L. J Drug Res1982; 32:230-235.