Nickel Enhances Collagen-induced Platelet Activation Acting by Increasing the Organization of the Cytoskeleton

Summary

In keratinocytes, osteoclasts and enterocytes, Ni²⁺ acts as an agonist working through selective activation of the polyvalent cation-sensing receptor.

We report here that while Ni²⁺ alone had no direct ability to induce platelet aggregation or secretion, Ni²⁺ pretreatment produced these responses when platelets were stimulated with subthreshold concentrations of collagen. In addition, pretreatment with Ni²⁺ significantly enhanced collagen-induced phospholipase C activation and calcium mobilization. Platelet adhesion to collagen was increased and the inhibition of collagen-induced adhesion normally seen after cytochalasin D treatment was significantly diminished.

When Ni²⁺ was added to platelets alone, tyrosine phosphorylation of p60^src was increased. Moreover, Ni²⁺ enhanced the amount of protein, especially actin, found in the low-speed Triton X-100 insoluble cyto-skeleton. Our results indicate that nickel, possibly acting via a platelet cation sensing receptor analogous to that which has been described in other cell types, may cause a rapid tyrosine kinase-dependent cyto-skeleton reorganization leading to enhanced adhesion of platelets to collagen and increasing collagen-dependent responses.

• References

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