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Am J Perinatol 2021; 38(S 01): e351-e358
DOI: 10.1055/s-0040-1710352
Original Article

Resveratrol Relieves Hyperoxia-Induced Brain Injury in Neonatal Rats by Activating Sirt1

Lan Kang
1   Department of Newborn Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China
,
Wenbin Dong
1   Department of Newborn Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China
,
Xiaobin Li
2   Department of Burns and Plastic Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China
,
Ying Ruan
1   Department of Newborn Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China
,
Rong Zhang
1   Department of Newborn Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China
› Author Affiliations Funding This study received its financial support from National Natural Science Foundation of China (grant number 81571480 to W.D.).
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Abstract

Objective Neonatal rats with hyperoxia-induced brain injury were treated with resveratrol to investigate its protective effects through analyzing changes in reactive oxygen species (ROS), Sirt1, p53, and acetylated p53 levels.

Study Design Neonatal rats were randomly divided into hyperoxia and resveratrol intervened groups. Rats in both groups were placed in a hyperoxia chamber for 7 days to induce hyperoxia-induced brain injury. The rats in the resveratrol intervened group were administered resveratrol 60 μg/g body weight daily, whereas those in the hyperoxia group were administered a dimethyl sulfoxide-based solvent. Brain tissues were collected, and hematoxylin and eosin (H&E) and TUNEL staining, ROS measurements, real time-polymerase chain reaction, and western blotting were performed.

Results H&E and TUNEL staining revealed increased cell damage and apoptosis in brain tissue from hyperoxia-exposed animals compared with the findings in animals in the resveratrol intervened group. Real time-polymerase chain reaction and western blotting identified increases in Sirt1 expression and decreases in p53 expression in the resveratrol intervened group. In addition, acetylated p53 protein expression was lower in the intervened group than in the hyperoxia group.

Conclusion Resveratrol alleviated brain apoptosis induced by hyperoxia in neonatal rats by upregulating Sirt1-mediated pathways, suggesting its potentially beneficial role in the treatment of brain injury induced by hyperoxia.

Keywords

Sirt1 - hyperoxia - brain injury - resveratrol


Publication History

Received: 11 December 2019

Accepted: 30 March 2020

Article published online:
01 May 2020

© 2020. Thieme. All rights reserved.

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