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Synfacts 2023; 19(08): 0825
DOI: 10.1055/s-0042-1751964
Innovative Drug Discovery and Development

Photoproximity Labeling of Proteins

Contributor(s):
Dirk Trauner
,
Tongil Ko
Hananya N, Ye X, Koren S, Muir TW. * Princeton University, USA
A genetically encoded photoproximity labeling approach for mapping protein territories.

Proc. Natl. Acad. Sci. 2023;
120: e2219339120
DOI: 10.1073/pnas.2219339120.
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Key words

photolabeling - LOV - proximity

Significance

Identifying direct and indirect interactions of proteins is an emerging area of interest in research. Tools that can match the dynamic spatial and temporal environment of cells are needed. The authors report a genetically encoded approach that allows for localized photolabeling of proteins with a cell-permeable probe.


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Comment

To achieve localized photolabeling, the authors fuse a Light-Oxygen-Voltage (LOV) domain containing a flavin molecule to their protein of interest (POI). This photoexcitable motif allows for the generation of singlet oxygen or SET to sensitize nearby amino acids or the small molecule probes BA and BP, allowing for their conjugation.


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Publication History

Article published online:
14 July 2023

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