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J Pediatr Infect Dis 2022; 17(04): 175-181
DOI: 10.1055/s-0042-1753463
Original Article

Evaluation of Salivary Thiol/Disulfide Homeostasis and Oxidative Stress in Children with Severe Early Childhood Caries Using a Novel Method

Asli Soğukpinar Önsüren
1   Department of Pediatric Dentistry, Faculty of Dentistry, Mersin University, Mersin, Turkey
,
Merve Mutluay
2   Vocational School of Health Services, Department of Dental Hygiene, Kırıkkale University, Kırıkkale, Turkey
,
Muhammed Seyithanoğlu
3   Department of Biochemistry, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey
,
Burak Tanriverdi
4   Department of Biochemistry, Artvin State Hospital, Artvin, Turkey
› Author Affiliations Funding This study was funded by Kahramanmaraş Sütçü İmam University Scientific Research Projects Coordination Unit (Project Number: 2020 /1-19M).
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Abstract

Objective This study aimed to assess the role of thiol/disulfide homeostasis and oxidative stress in the saliva of children with severe early childhood caries (S-ECC).

Methods Eighty children aged 3 to 6 years were involved in this case-control study. The study consisted of two groups: the study group (S-ECC) and the control group with no caries. Thiol/disulfide homeostasis and antioxidant levels were calculated after obtaining unstimulated saliva samples from all participating children.

Results The native/total thiol and total oxidant status (TOS) levels of the study group were higher than those of the control group, though not statistically significant (p > 0.05). The oxidative stress index (OSI) value was significantly higher in the study group compared to the control group (p = 0.024).

Conclusion Our results confirmed that the thiol/disulfide homeostasis was reduced, and disulfide formation, which is rereducible to thiol, was insufficient in children with S-ECC to compensate oxidative stress compared with the control group. Also, thiol levels were inadequate to compensate for oxidative stress, and thiol/disulfide homeostasis was not an independent parameter for S-ECC. Besides, the increases in the TOS level and OSI value show that oxidative stress had significant effects on S-ECC's etiopathogenesis.

Keywords

native thiol - thiol-disulfide homeostasis - total antioxidant status


Publication History

Received: 22 January 2022

Accepted: 25 May 2022

Article published online:
02 August 2022

© 2022. Thieme. All rights reserved.

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