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Gesundheitswesen 2000; 62(11): 604-608
DOI: 10.1055/s-2000-13042
© Georg Thieme Verlag Stuttgart · New York

Verfolgung von Gruppenerkrankungen mit Norwalk-like-Viren (NLV) in Baden-Württemberg

Isolierung und Detektion von Norwalk-like-Virus-RNA aus Stuhlproben mittels RT-PCRSurveillance of Norwalk associated group infections in Baden-Württemberg. Isolation and detection of Norwalk-RNA from stool samples by RT-PCRJ. Fleischer, C. Wagner Wiening, P. Kimmig
  • Landesgesundheitsamt Baden-Württemberg
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Publication History

Publication Date:
31 December 2000 (online)

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Zusammenfassung

In den Jahreswechseln 98/99 und 99/2000 wurden von mehreren Gesundheitsämtern in Baden-Württemberg im Rahmen von Gruppenerkrankungen zahlreiche Stuhlproben zur Untersuchung auf gastrointestinale Erreger an das Landesgesundheitsamt Baden-Württemberg eingesandt. Während die bakteriologischen Routineuntersuchungen sowie Virus-Antigen-Untersuchungen auf Adeno-, Astro- und Rotaviren negativ blieben, ergaben zusätzliche RT-PCR-Untersuchungen auf Norwalkviren vermehrt positive Befunde. Insgesamt wurden im LGA im Zeitraum von Dezember 98 bis Januar 2000 aus 14 Gruppenerkrankungen 286 Stuhlproben mittels RT-PCR untersucht, wobei in 118 Fällen Norwalk-Virus-RNA nachgewiesen werden konnte. Zur Verifizierung der Analysen wurden Stichproben von einzelnen Gruppenerkrankungen an das Robert-Koch-Institut, Abt. Molekulare Virologie, verschickt; hier wurde der Befund „Norwalk-Viren” bestätigt.

Surveillance of Norwalk associated group infections in Baden-Württemberg. Isolation and detection of Norwalk-RNA from stool samples by RT-PCR

Between the turn of the years 98/99 and 99/2000, stool samples from various health departments in the south of Germany were sent to the LGA (Landesgesundheitsamt Baden-Württemberg). Different testings as bacteriological routines for enterobacteriaceae and virological antigen screenings of the stool samples for adeno-, astro- and rotaviruses lead to negative results. Additional RT-PCR tests for the presence of Norwalk-RNA showed nearly 40 % of positive samples. From 14 group infections between december 98 and january 2000, 286 samples in total were sent to the LGA, 118 of them gave positive results for the norwalk agent. For the verification of the analysis, some random samples were sent to the Robert-Koch-Institut, div. of molecular virology at Berlin, where the results were confirmed.

Keywords

Norwalk-Like-Virus(NLV) - Virus-RNA - Norwalk-Associated Groupinfections - Stool Samples - RT-PCR

Literatur

  • 1 Kaplan J E. et al . Epidemiology of Norwalk gastroenteritis and the role of Norwalk virus in outbreaks of acute nonbacterial gastroenteritis.  Ann Int Med. 1982;  96 756-761
    PubMedGoogle Scholar
  • 2 Green K Y. The role of human caliciviruses in epidemic gastroenteritis.  Arch Virol. 1997;  13 153-165
    PubMedGoogle Scholar
  • 3 Robert-Koch-Institut .Zur Situation bei wichtigen Infektionskrankheiten im Jahr 1998 Teil 1: Darminfektionen (Gastroenteritiden). Epid. Bull 1999 15/99
    Google Scholar
  • 4 Centers For Disease Control And Prevention .Viral agents of gastroenteritis: public health and outbreak management. Morbd. Mortal. Weekly Rep 39 1990 RR-5: 1-24
    Google Scholar
  • 5 Centers For Disease Control And Prevention .Norwalk like viral gastroenteritis in U.S. Army trainees - Texas, 1998. Morbid. Mortal. Weekly Rep 48 1999 11
    Google Scholar
  • 6 Moe C L, Gentsch J, Ando T, Grohmann G, Monroe S S, Jiang X, Wang J, Estes M K, Seto Y, Humphrey C, Stine S, Glass R I. Application of PCR to detect norwalk virus in fecal specimens from outbreaks of gastroenterits.  J Clin Microbiol. 1994;  32 642-648
    PubMedGoogle Scholar
  • 7 Parker S P, Cubitt W D, Jiang X J, Estes M K. Seroprevalence studies using a recombinant norwalk virus protein enzyme immunoassay.  J Med Virol. 1994;  42 146-150
    PubMedGoogle Scholar
  • 8 Willcocks M M, Silcock J G, Carter M J. Detection of norwalk virus in the UK by polymerase chain reaction.  FEMS Microbiol. 1993;  Letters 112 7-12
    PubMedGoogle Scholar
  • 9 Kapikian A Z, Estes M K, Chanock R M. Norwalk Group of Viruses. Fields N, Knipe DM et al Virology, Third Edition Raven Press Ltd. New York 1996 25: 783-810
    Google Scholar
  • 10 Lawson H W, Braun M M, Glass R IM, Stine S E, Monroe S S, Atrasch H K, Lee L E, Englender S J. Waterbone outbreak of Norwalk virus gastroenteritis at a southwest US resort: role of geological formations in contamination of well water.  Lancet. 1991;  337 1200-1204
    CrossrefPubMedGoogle Scholar
  • 11 Payment P, Franco E. Incidence of Norwalk viruses infections during a prospective epidemiological study of drinking water related gastrointestinal illness.  Can J Microbiol. 1994;  40 805-809
    PubMedGoogle Scholar
  • 12 Schwab K J, Estes M K, Neill F H, Atmar R L. Use of heat release and an internal RNA standard control in reverse transcription-PCR detection of Norwalk virus from stool samples.  J Clinic Microbiol. 1997;  35 (2) 511-514
    PubMedGoogle Scholar
  • 13 Chadwick P R, Walker M, Rees A E. Airborne transmission of a small round structured virus.  Lancet. 1994;  343 171
    PubMedGoogle Scholar
  • 14 Kapikian A Z, Wyatt R G, Dolin R, Thornhill T S, Kalica A R, Channock R M. Visualization by immune electron microscopy of a 27nm particle associated with a non-bacterial gastroenteritis.  J Virol. 1972;  10 1075-1081
    PubMedGoogle Scholar
  • 15 Dolin R, Blacklow N R, Dupont H, Buscho R S, Wyatt R G, Kasel J A, Hornick R, Channock R M. Biological properties of Norwalk agent of acute infectious nonbacterial gastroenteritis.  Proc Soc Exp Biol Med. 1972;  140 578-583
    PubMedGoogle Scholar
  • 16 Saif L J, Bohl E H, Theil K W, Cross R F, House J A. Rotavirus-like, calicivirus-like and 23nm virus-like particles associated with diarrhea in young pigs.  J Clin Microbiol. 1980;  12 105-111
    PubMedGoogle Scholar
  • 17 Woode G N, Bridger J C. Isolation of small viruses resembling astroviruses and caliciviruses from acute gastroenteritis of calves.  J Med Microbiol. 1978;  11 441-425
    PubMedGoogle Scholar
  • 18 Caul E O, Appleton H. The electron microscopical and physical characteristics of small round human fecal viruses: an interim scheme for classification.  J Med Virol. 1982;  9 257-265
    PubMedGoogle Scholar
  • 19 Dolin R, Reichmann R C, Roessner K D, Tralka T S, Schoely R T, Gary W, Morens D. Detection by immune electron microscopy of the Snow Mountain agent of acute viral gastroenteritis.  J Infect Dis. 1982;  146 184-189
    PubMedGoogle Scholar
  • 20 Lambden P R, Caul E O, Ashley C R, Clarke I N. Human enteric caliciviruses are generally distinct from small round structured viruses.  Lancet. 1994;  34 666-667
    PubMedGoogle Scholar
  • 21 Wyatt. et al . Comparison of three agents of acute infectious nonbacterial gastroenteritis by cross-challenge in volunteers.  J Infect Dis. 1974;  129 709-714
    PubMedGoogle Scholar
  • 22 Jiang X, Graham D, Wang K, Estes M K. Norwalk virus genome cloninig and characterisation.  Science. 1990;  250 1580-1583
    PubMedGoogle Scholar
  • 23 Jiang X, Wang K, Wang M, Estes M K. Sequence and genomic organisation of Norwalk virus.  Virology. 1993;  95 51-61
    PubMedGoogle Scholar
  • 24 Carter M J, Milton I D, Madeley C R. Caliciviruses.  Rev Med Virol. 1991;  1 177-186
    PubMedGoogle Scholar
  • 25 Gary G W JR, Anderson L J, Keswick B N, Johnson P C, Dupont H, Stine S E, Bartlet A V. Norwalk virus antigen and antibody response in an adult volunteer study.  J Clin microbiol. 1987;  25 2001-2003
    PubMedGoogle Scholar
  • 26 Okada S, Sekine S, Ando T, Hayashi Y, Murao M, Yabuuchi K, Miki T, Ohashi M. Antigenic characterisation of small, round-structured viruses by immune electron microscopy.  J Clinic Microbiol. 1990;  28 1244-1248
    PubMedGoogle Scholar
  • 27 Schreier E, Döring F, Künkel U. Molecular epidemiology of outbreaks of gastroenteritis associated with small round structured viruses in Germany 1997/98.  Arch Virol. 2000;  145 1-11
    PubMedGoogle Scholar
  • 28 Betram S, Gassen H G. Gentechnische Methoden, Eine Sammlung von Arbeitsanleitungen für das molekularbiologische Labor. G. Fischer Verlag 1991
    Google Scholar
  • 29 Boom. et al . Rapid and simple Method for the purification of nucleic acids.  J Clin Microbiol. 1990;  28 495-503
    PubMedGoogle Scholar
  • 30 Maniatis T, Fritsch E F, Sambrook J. Molecular Cloning: A laboratory manual. Cold Spring Harbour Laboratory. Cold Spring Harbour N.Y; 1989
    Google Scholar
  • 31 Schwab K J, Leon R DE, Sobsey M D. Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk by reverse transcription-PCR.  Appl Environ Microbiol. 1995;  61 531-537
    PubMedGoogle Scholar
  • 32 Fleischer J, Kimmig P. Ausbruch von Norwalk-Virus-Infektionen in einem Seniorenheim. Epid. Bull 1999 29/99: 213-214.
    Google Scholar
  • 33 Steingaß S, Walter K, Knoblauch H, Kimmig P, Fleischer J. Gastroenteritis-Ausbruch durch Norwalk-Viren in einem Pflegeheim. Epid. Bull 1999 29/99
    Google Scholar
  • 34 Robert-Koch-Institut .Zur hygienischen Händedesinfektion bei Virusinfektionen. Epid. Bull 1999 29/99: 216
    Google Scholar
  • 35 Pönkä A, Maunula L, Bonsdorff C H von, Lyytikäinen O. Outbreak of calicivirus gastroenteritis associated with eating frozen raspberries.  Euro surveillance, Vol. 4. 1999;  6 66-69
    PubMedGoogle Scholar
  • 36 Robert-Koch-Institut .Übersicht: Gastroenteritis durch Norwalk- und Norwalk-like-Viren. Epid. Bull 1998 6/98
    Google Scholar

Dr. J. Fleischer

Landesgesundheitsamt Baden-Württemberg

Wiederholdstraße 15

70174 Stuttgart

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