Effects of Human Chorionic Gonadotropin on Trophoblast Invasion

ABSTRACT

Recent studies have shown that different populations of trophoblastic cells of first-trimester human placenta express human chorionic gonadotropin/luteinizing hormone (hCG/LH) receptors. Among these trophoblastic cells, extravillous cytotrophoblasts (CTBs) not only express hCG/LH receptors but also present an invasive phenotype. CTB invasion is instrumental in blastocyst implantation and later in placentation and is tightly regulated in both time and space. This article describes some of the regulators of CTB invasion and summarizes and discusses the potential autocrine/paracrine role of hCG in trophoblast invasion.

KEYWORD

hCG - trophoblast invasion - metalloproteinases - integrins

REFERENCES

• 1 Mochizuki M. Trophoblast: its functional regulation and pathophysiological profiles.  Nippon Sanka Fujinka Gakkai Zasshi . 1992;  44 918-928
• 2 Horn L C, Emmrich P, Bilek K. The early placental trophoblast: I. Normal development and endometrial and-tumour-induced disorders.  Zentralbl Gynakol . 1996;  118 487-497
• 3 Blankenship T N, Enders A C, King B F. Trophoblastic invasion and modification of uterine veins during placental development in macaques.  Cell Tissue Res . 1993;  274 135-144
• 4 Yagel S, Parhar R S, Jeffrey J J, Lala P K. Normal nonmetastatic human trophoblast cells share in vitro invasive properties of malignant cells.  J Cell Physiol . 1988;  136 455-462
• 5 Yagel S, Geva T E, Solomon H. High levels of hCG retard first trimester trophoblast invasion in vitro by decreasing urokinase plasminogen activator and collagenase activities.  J Clin Endocrinol Metab . 1993;  77 1506-1511
• 6 Bischof P, Martelli M, Campana A. The regulation of endometrial and trophoblastic metalloproteinases during blastocyst implantation.  Contracept Fertil Sex . 1994;  22 48-52
• 7 Morgan M, Kniss D, McDonnell S. Expression of metalloproteinases and their inhibitors in human trophoblast continuous cell lines.  Exp Cell Res . 1998;  242 18-26
• 8 Murphy G, Atkinson S, Ward R, Gavrilovic J, Reynolds J J. The role of plasminogen activators in the regulation of connective tissue metalloproteinases.  Ann N Y Acad Sci . 1992;  667 1-12
• 9 Ogata Y, Enghild J J, Nagase H. Matrix metalloproteinase-3 (stromelysin) activates the precursor for human matrix metalloproteinase-9.  J Biol Chem . 1992;  267 3581-3584
• 10 Bischof P, Campana A. A model for implantation of the human blastocyst and early placentation.  Hum Reprod Update . 1996;  2 262-270
• 11 Sato H, Takino T, Okada Y. A matrix metalloproteinases expressed on the surface of invasive tumour cells.  Nature . 1994;  370 61-65
• 12 Polette M, Nawrocki B, Pintiaux A. Expression of gelatinases A and B and their tissue inhibitors by cells of early and term human placenta and gestational endometrium.  Lab Invest . 1994;  71 838-846
• 13 Fisher S J, Cui T, Zhang L. Adhesive and degradative properties of human placental cytotrophoblast cells in vitro.  J Cell Biol . 1989;  109 891-902
• 14 Bischof P, Friedli E, Martelli M, Campana A. Expression of extracellular matrix-degrading metalloproteases by cultured human cytotrophoblast cells: effect of cell adhesion and immunopurification.  Am J Obstet Gynecol . 1991;  65 1791-1801
• 15 Bischof P, Haenggeli L, Campana A. Gelatinase and oncofetal fibronectin secretion are dependent upon integrin expression on human cytotrophoblasts.  Hum Reprod . 1995;  10 734-742
• 16 Bischof P, Martelli M, Campana A, Itoh Y, Ogata Y, Nagase H. Importance of metalloproteinases (MMP) in human trophoblast invasion.  Early Pregnancy Biol Med . 1995;  1 263-269
• 17 Librach C L, Werb Z, Fitzgerald M L. 92 kDa type IV collagenase mediates invasion of human cytotrophoblasts.  J Cell Biol . 1991;  113 437-449
• 18 Lala P K, Graham C H. Mechanisms of trophoblast invasiveness and their control: the role of proteases and protease inhibitors.  Cancer Metastasis Rev . 1990;  9 369-379
• 19 Loke Y W, Burrows T, King A. Adhesion molecules and trophoblastic invasion.  Contracept Fertil Sex . 1995;  23 573-575
• 20 Bischof P, Meisser A, Campana A. Mechanisms of endometrial control of trophoblast invasion.  J Reprod Fertil . 2000;  5(suppl) 65-71
• 21 Graham C H, Lala P K. Mechanisms of placental invasion of the uterus and their control.  Biochem Cell Biol . 1992;  70 867-874
• 22 Murphy G, Docherty A J. The matrix metalloproteinases and their inhibitors.  Am J Respir Cell Mol Biol . 1992;  7 120-125
• 23 Bischof P, Meisser A, Campana A, Tseng L. Effects of decidua conditioned medium and insulin-like growth factor binding protein-1 on trophoblast matrix metalloproteinases and their inhibitors.  Placenta . 1998;  19 457-464
• 24 Hamilton G S, Lysiak J J, Han V K, Lala P K. Autocrine-paracrine regulation of human trophoblast invasiveness by insulin-like growth factor (IGF)-II and IGF-binding protein (IGFBP)-1.  Exp Cell Res . 1998;  10 147-156
• 25 Irwin J C, Giudice L C. Antibody to insulin-like growth factor binding protein-1 (IGFBP-1) stimulates cytotrophoblast invasion into decidualised endometrial stromal cultures.  J Soc Gynecol Invest . 1997;  4 103A
• 26 Lustbader J W, Lobel L, Wu H, Elliott M M. Structural and molecular studies of hCG and its receptor.  Recent Prog Horm Res . 1998;  53 395-424
• 27 Rabe T, Scheurer A, Muller A, Runnenbaum B. Pharmacologically induced changes in beta-hCG synthesis by human placenta in organ culture.  Int J Biol Res Pregnancy . 1982;  3 139-147
• 28 Cemerikic B, Zamah R, Ahmed M S. Opioids regulation of hCG release from trophoblast tissue is mediated by gonadotropin releasing hormone.  J Pharmacol Exp Ther . 1994;  268 971-977
• 29 Lin L S, Roberts V J, Yen S S. Expression of human gonadotropin-releasing hormone receptor gene in the placenta and its functional relationship to hCG secretion.  J Clin Endocrinol Metab . 1995;  80 580-585
• 30 Mochizuki M, Maruo T, Matsuo H, Samoto T, Ishihara N. Biology of human trophoblast.  Int J Gynaecol Obstet . 1998;  60(suppl 1) S21-S28
• 31 Tao Y X, Lei Z M, Hofmann G E, Rao C V. Human intermediate trophoblasts express chorionic gonadotropin/luteinising hormone receptor gene.  Biol Reprod . 1995;  53 899-904
• 32 Licht P, Cao H, Zuo J. Lack of self-regulation of human chorionic gonadotropin biosynthesis in human choriocarcinoma cells.  J Clin Endocrinol Metab . 1994;  78 1188-1194
• 33 Lei Z M, Rao C V, Ackerman D M, Day T G. The expression of human chorionic gonadotropin/human luteinising hormone receptors in human gestational trophoblastic neoplasms.  J Clin Endocrinol Metab . 1992;  74 236-241
• 34 Zygmunt M, Hahn D, Munstedt K, Bischof P, Lang U. Invasion of cytotrophoblastic JEG-3 cells is stimulated by hCG in vitro.  Placenta . 1998;  19 587-593
• 35 Milwidsky A, Finci-yeheslek Z, Yagel S, Mayer M. Gonadotropin-mediated inhibition of proteolytic enzymes produced by human trophoblast in culture.  J Clin Endocrinol Metab . 1993;  76 1101-1105
• 36 Oktay K, Brzyski R G, Miller E B, Krugman D. Association of serum beta-hCG levels with myosalpingeal invasion and viable trophoblast mass in tubal pregnancy.  Obstet Gynecol . 1994;  84 803-806
• 37 Klein M, Graf A, Kiss H. The relation between depth of trophoblastic invasion and beta-hCG levels in tubal pregnancies.  Arch Gynecol Obstet . 1995;  256 85-88
• 38 McIntush E W, Smith M F. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in ovarian function.  Rev Reprod . 1998;  3 23-30
• 39 Aston K E, Stamouli A, Thomas E J. Effect of gonadotrophin on cell and matrix retention and expression of metalloproteinases and their inhibitor in cultured human granulosa cells modelling corpus luteum function.  Mol Hum Reprod . 1996;  2 26-30
• 40 Duncan W C, McNeilly A S, Illingworth P J. The effect of luteal ``rescue'' on the expression and localisation of matrix metalloproteinases and their tissue inhibitors in human corpus luteum.  J Clin Endocrinol Metab . 1998;  83 2470-2478
• 41 O'Sullivan M J, Stamouli A, Thomas E J, Richardson M C. Gonadotrophin regulation of production of tissue inhibitor of metalloproteinases-1 by luteinised human granulosa cells: a potential mechanism for luteal rescue.  Mol Hum Reprod . 1997;  3 405-410
• 42 Tadakuma H, Okamura H, Kitaoka M, Iyama K, Usuku G. Association of immunolocalisation of matrix metalloproteinase 1 with ovulation in hCG-treated rabbit ovary.  J Reprod Fertil . 1993;  98 503-508
• 43 Bagavandoss P. Differential distribution of gelatinases and tissue inhibitor of metalloproteinase-1 in the rat ovary.  J Endocrinol . 1998;  158 221-228
• 44 Stamouli A, O'Sullivan M J, Frankel S, Thomas E J, Richardson M C. Suppression of matrix metalloproteinase production by HCG in cultures of human luteinised granulosa cells as a model for gonadotrophin-induced luteal rescue.  J Reprod Fertil . 1996;  107 235-239
• 45 Duncan W C, McNeilly A S, Illingworth P J. Expression of tissue inhibitor of metalloproteinases-1 in the human corpus luteum after luteal rescue.  J Endocrinol . 1996;  148 59-67