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Synfacts 2005(1): 0181-0181  
DOI: 10.1055/s-2005-916024
Polymer-Supported Synthesis
© Georg Thieme Verlag Stuttgart · New York

PGA-Catalyzed Hydrolysis of Phenylacetic Hydrazides on a Solid Phase

Contributor(s): Yasuhiro Uozumi, Yoichi M. A. Yamada, Naoshi Fukuyama
A. Basso, C. Ebert, L. Gardossi*, P. Linda, T. T. Phuong, M. Zhu, L. Wessjohann*
Università degli Studi Trieste, Italy and Leibniz Institute of Plant Biochemistry, Halle, Germany
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Publication History

Publication Date:
21 September 2005 (online)

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Significance

Phenylacetic hydrazide which was chemically synthesized on PEGA1900 or PEGA+ (Chem. Commun. 2003, 1296-1297) was enzymatically hydrolyzed at room temperature (24 h) by penicillin G amidase (PGA) to release phenyl­acetic acid. Release of phenylacetic acid from PEGA­+-bound phenylacetic hydrazide in 0.01 M Kpi buffer was almost 60%, while that from PEGA1900-bound phenylacetic hydrazide was 20%.