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Endoscopy 2007; 39(6): 525-529
DOI: 10.1055/s-2007-966391
Original article

© Georg Thieme Verlag KG Stuttgart · New York

The utility of contrast-enhanced endoscopic ultrasound in monitoring ethanol-induced pancreatic tissue ablation: a pilot study in a porcine model

S.  A.  Giday1 , P.  Magno1 , K.  L.  Gabrielson2 , J.  M.  Buscaglia1 , M.  I.  Canto1 , C.  W.  Ko3, 4 , J.  O.  Clarke1 , A.  N.  Kalloo1 , S.  B.  Jagannath1 , E.  J.  Shin1 , S.  V.  Kantsevoy1
  • 1Department of Medicine, Division of Gastroenterology, Johns Hopkins Hospital, Baltimore, Maryland, USA
  • 2Department of Molecular and Comparative Pathobiology, Johns Hopkins University, Baltimore, Maryland, USA
  • 3Division of Gastroenterology, Taichung Veterans General Hospital, Taichung, Taiwan
  • 4Division of Gastroenterology, National Yang-Ming University, Taipei, Taiwan
Further Information

Publication History

submitted 22 December 2006

accepted after revision 13 March 2007

Publication Date:
06 June 2007 (online)

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Commentaire de travail de S. A. Giday et al., pp. 525Endoscopy 2007; 39(06): 578-578
DOI: 10.1055/s-0032-1306945

Background and study aims: Pancreatic ablation is gaining popularity for the treatment of focal pancreatic lesions. The aim of our study was to evaluate local effects of intrapancreatic alcohol injection and the utility of contrast-enhanced endoscopic ultrasound (EUS) for its monitoring in a porcine model. Methods: We performed four survival experiments on 50-kg pigs. Under linear EUS guidance, 0.5mL of 50 % ethanol plus purified carbon particle solution (GI Spot®) was injected into the pancreatic body to create a focal area of pancreatic necrosis. The animals survived for 24 - 48 hours (pigs # 1, # 2, and # 3) and 7 days (pig # 4). EUS was then repeated with and without perflutren lipid microspheres (Definity®) administration through the peripheral vein. Standard and microsphere-enhanced images of the pancreas were compared. Afterwards the animals were euthanized for necropsy. Results: Alcohol injection caused focal pancreatic necrosis, which was barely seen by standard EUS as a subtle hypoechoic lesion 1 cm in diameter. Color and power Doppler EUS of this region did not reveal any blood flow. After intravenous injection of microspheres, color Doppler EUS revealed marked contrast enhancement of normal pancreatic parenchyma with a clearly delineated avascular alcohol-treated area, which on postmortem examination corresponded to the discrete necrotic area marked with carbon particles. Conclusions: EUS-guided alcohol injection consistently causes focal areas of pancreatic necrosis. Contrast-enhanced EUS with microspheres improves visualization of altered pancreatic vascular perfusion and can be used to facilitate detection of small pancreatic lesions and its follow-up post-ablation.

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S. V. Kantsevoy, MD 

Johns Hopkins Hospital
Division of Gastroenterology

1830 East Monument Street Room 423
Baltimore, MD 21205
USA

Fax: +1-410-614-2490

Email: svkan@jhmi.edu

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