Phlebologie 2016; 45(02): 100-105
DOI: 10.12687/phleb2303-2-2016
Review Article
Schattauer GmbH

Plastic chronic wound management with Cellutome

An old/new technique in autologous skin transplantation Article in several languages: English | deutsch
G. U. Seip
1   St. Marien- und St. Annastiftskrankenhaus Ludwigshafen, Germany
› Author Affiliations
Further Information

Publication History

Received: 29 January 2016

Accepted: 01 February 2016

Publication Date:
21 December 2017 (online)

Summary

Chronic wounds continue to present a significant challenge to health-care providers around the globe. Unlike acute wounds, chronic wounds do not proceed through an orderly process of repair. In recent years many new modalities of modern wound treatment systems have been promoted. However, until recently there were few modalities designed to promote epithelialisation of a fully granulated wound. Mesh graft procedures have long been the gold standard for the management of acute wounds and chronic wounds but have also many disadvantages like discomfort associated with the donor site and the creation of a second painful wound (donor site).

The increase of chronical wounds in Germany due to the average age of patients, the aggressiveness of medical treatment and increase of numbers of patients with diabetes and severe polymorbidity requires specialized wound treatment and plastic surgery. Since 2014 there was a new innovative system introduced in the market called Cellutome epidermal harvesting system. The Cellutome system is a epidermal harvesting system for skin grafting and can replace in many cases the traditional meshgraft procedure with a classic dermatoma. The skin donor section on the patient`s thigh heals within days without scarring. The system offers a precise, simplified and minimal invasive option for skin grafting in the treatment of especially chronic wounds.

 
  • References

  • 1 Osborne SN, Schmidt MA, Derrick K, Harper JR. Epidermal micrografts produced via an automated and minimally invasive tool form at the dermal/epidermal junction and contain proliferative cells that secrete wound healing growth factors. Adv Skin Wound Care 2015; 28 (09) 397-405 doi: 10.1097/ 01.ASW.0000470024.81711.b8. PMID: 26258460.
  • 2 Osborne SN, Schmidt MA, Harper JR. An Automated and Minimally Invasive Tool for Generating Autologous Viable Epidermal Micrografts. Adv Skin Wound Care 2016; 29 (02) 57-64 doi: 10.1097/01.ASW.0000476072.88818.aa. PMID: 26765157.
  • 3 Serena TE. Use of epidermal grafts in wounds: a review of an automated epidermal harvesting system. J Wound Care 2015; 24 (4 Suppl): 30-34 doi: 10.12968/jowc.2015.24.Sup4b.30. PMID: 25853646.
  • 4 Serena TE. The increasing role of epidermal grafting utilizing a novel harvesting system in chronic wounds. Wounds 2015; 27 (02) 26-30 PMID: 25785907.
  • 5 Gabriel A, Sobota RV, Champaneria M. Initial experience with a new epidermal harvesting system: overview of epidermal grafting and case series. Surg Technol Int 2014; 25: 55-61 PMID: 25433225.
  • 6 Spiekstra SW, Breetveld M, Rustemeyer T. et al. Wound-healing factors secreted by epidermal keratinocytes and dermal fibroblasts in skin substitutes. Wound Repair Regen 2007; 15 (05) 708-717 PMID:17971017.
  • 7 Pertusi G, Tiberio R, Graziola F. et al. Selective release of cytokines, chemokines, and growth factors by minced skin in vitro supports the effectiveness of autologous minced micrografts technique for chronic ulcer repair. Wound Repair Regen 2012; 20 (02) 178-184 doi: 10.1111/j.1524–475X. 2011.00762.x. Epub 2012 Feb 3. PMID: 22304391.
  • 8 Serena T, Francius A, Taylor C, MacDonald J. Use of a novel epidermal harvesting system in resource-poor countries. Adv Skin Wound Care 2015; 28 (03) 107-112 doi: 10.1097/01.ASW. 0000460839.72826.ce. PMID: 25658643.
  • 9 Xian LJ, Chowdhury SR, Bin ASaim, Bt Hj RIdrus. Concentration-dependent effect of platelet-rich plasma on keratinocyte and fibroblast wound healing. Cytotherapy 2015; 17 (03) 293-300 doi: 10.1016/j.jcyt.2014.10.005. Epub 2014 Nov 21. PMID: 25456581.
  • 10 Amano S, Akutsu N, Ogura Y, Nishiyama T. Increase of laminin 5 synthesis in human keratinocytes by acute wound fluid, inflammatory cytokines and growth factors, and lysophospholipids. Br J Dermatol 2004; 151 (05) 961-970 PMID: 15541073.
  • 11 El Ghalbzouri A, Hensbergen P, Gibbs S. et al. Fibroblasts facilitate re-epithelialization in wounded human skin equivalents. Lab Invest 2004; 84 (01) 102-112 PMID: 14631386.
  • 12 Hebda PA. Stimulatory effects of transforming growth factor-beta and epidermal growth factor on epidermal cell outgrowth from porcine skin explant cultures. J Invest Dermatol 1988; Nov; 91 (05) 440-445 PMID: 3262693.
  • 13 Satish L, Yager D, Wells A. Glu-Leu-Arg-negative CXC chemokine interferon gamma inducible protein-9 as a mediator of epidermal-dermal communication during wound repair. J Invest Dermatol 2003; 120 (06) 1110-1117 PMID: 12787142.
  • 14 El-Ghalbzouri A, Van Den Bogaerdt AJ, Kempenaar J, Ponec M. Human adipose tissue-derived cells delay re-epithelialization in comparison with skin fibroblasts in organotypic skin culture. Br J Dermatol 2004; 150 (03) 444-454 PMID: 15030326.
  • 15 Purschke M, Asrani FA, Sabir SA. et al. Novel methods for generating fractional epidermal micrografts. Br J Dermatol 2015; Apr; 172 (04) 1021-1028.
  • 16 Krugluger W, Rohrbacher W, Moser K. et al. Induction of vascular endothelial growth factor messenger ribonucleic acid expression in stored micrografts by aminoguanidine. Dermatol Surg 2005; 31 (11 Pt 1): 1404-1408 PMID: 16416608.
  • 17 Vogt PM, Lehnhardt M, Wagner D. et al. Determination of endogenous growth factors in human wound fluid: temporal presence and profiles of secretion. Plast Reconstr Surg 1998; 102 (01) 117-123.
  • 18 Mann A, Breuhahn K, Schirmacher P, Blessing M. Keratinocyte-derived granulocyte-macrophage colony stimulating factor accelerates wound healing: Stimulation of keratinocyte proliferation, granulation tissue formation, and vascularization. J Invest Dermatol 2001; 117 (06) 1382-1390.
  • 19 Varkey M, Ding J, Tredget EE. Superficial dermal fibroblasts enhance basement membrane and epidermal barrier formation in tissue-engineered skin: implications for treatment of skin basement membrane disorders. Tissue Eng Part A 2014; 20 (3–4): 540-552.
  • 20 Ferguson MW, O’Kane S. Scar-free healing: from embryonic mechanisms to adult therapeutic intervention. Philos Trans R Soc Lond B Biol Sci 2004; May 29; 359 (1445): 839-850 Review. PMID: 5293811.