Download PDF
Vet Comp Orthop Traumatol 1998; 11(03): 136-140
DOI: 10.1055/s-0038-1632535
Original Research
Schattauer GmbH

The Effect of Polysulphated Glycosaminoglycan on the Production of Proteoglycan by Equine Tendon Fibroblasts in Monolayer Culture

Linda A. Dahlgren
1   From the Department of Veterinary Clinical Sciences, Ames, Iowa, USA
,
R. F. Rosenbusch
2   Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
,
L. C. Booth
1   From the Department of Veterinary Clinical Sciences, Ames, Iowa, USA
› Author Affiliations
Further Information

Publication History

Received:09 October 1996

Accepted:01 December 1997

Publication Date:
10 February 2018 (online)

    Permissions and Reprints

Summary

Equine tendon fibroblasts were isolated from tissue fragments of superficial digital flexor tendon, subcultured and maintained in monolayers. The cells were treated with two dosage regimes of polysulphated glycosaminoglycan (PSGAG). The high dose group received 0,25, 50 or 200 μg/ml of PSGAG and the low dose group received 0,0.3,1 or 2 μg/ml of PSGAG. Proteoglycan production was measured via liquid scintillation counting, following radiolabel with 35SO4 (40 μCi/ml). The counts were measured in both the cell associated fraction and the supernatant fraction.

The addition of PSGAG to the culture system did not adversely affect cell viability at any treatment level. The cells in all of treatment groups produced measurable amounts of proteoglycan in both the cell associated and supernatant fractions. There were not any differences between the treatment means in any of the four groups (high dose cell-associated, high dose supernatant, low dose cell-associated, low dose supernatant). A linear trend in proteoglycan production was not documented between treatment groups in either the cell-associated or the supernatant fractions for either of the two dosage regimes. The significance of the lack of effect is equivocal. PSGAG may influence tendon healing in a manner other than by increasing proteoglycan production.

The effect of polysulphated glycosaminoglycan on the production of proteoglycans by equine tendon fibroblasts was investigated. A previously described model was utilized to grow the tendon fibroblasts in monolayer cultures. Significant treatment effects were not seen at either a high or low dose of PSGAG. Addition of the PSGAG did not adversely affect cell viability at any treatment level.

Keywords

Tendinitis - polysulphated glycosaminoglycan
 

  • REFERENCES

  • 1 Altman RD, Dean DD, Muniz OE, Howell DS. Prophylactic treatment of canine osteoarthritis with glycosaminoglycan polysulfuric acid ester. Arthritis Rheum 1989; 32: 759-66.
    CrossrefPubMedGoogle Scholar
  • 2 Baici A, Salgam P, Fehr K, Boni A. Inhibition of human elastase from polymorphonuclear leucocytes by a glycosaminoglycan polysulphate (Arteparon®). Biochem Pharmacol 1980; 29: 1723-7.
    CrossrefPubMedGoogle Scholar
  • 3 Brennan JJ, Aherne FX, Nakano T. Effects of glycosaminoglycan polysulphate treatment on soundness, hyaluronic acid content of synovial fluid and proteoglycan aggregate in articular cartilage of lame boars. Can J Vet Res 1987; 51: 394-8.
    PubMedGoogle Scholar
  • 4 Burba DJ, Collier MA, Default LE, Hanson- Painton O, Thompson HC, Holder CL. In vivo kinetic study on uptake and distribution of intramuscular tritium-labeled polysulphated glycosaminoglycan in equine body fluid compartments and articular cartilage in an osteochondral defect model. J Eq Vet Sci 1993; 13: 696-703.
    CrossrefPubMedGoogle Scholar
  • 5 Burkhardt D, Ghosh P. Laboratory evaluation of glycosaminoglycan polysulphate ester for chondroprotective activity: A review. Current Ther Res 1986; 40: 1034-52.
    PubMedGoogle Scholar
  • 6 Caron JP, Eberhart SW, Nachreiner R. Influence of polysulphated glycosaminoglycan on equine articular cartilage in explant culture. Am J Vet Res 1991; 52: 1622-25.
    PubMedGoogle Scholar
  • 7 Caron JP, Toppin DS, Block JA. Effect of polysulphated glycosaminoglycan on osteoarthritic equine articular cartilage in explant culture. Am J Vet Res 1993; 54: 1116-21.
    PubMedGoogle Scholar
  • 8 Dahlgren LA, Rosenbusch RF, Booth LC. Development of an in vitro model for the study of the response of equine tendon fibroblasts to injury and medication. Vet Comp Orthop Traumatol 1997; 10: 6-11.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 9 Fubini SL, Boatwright CE, Todhunter RJ, Lust G. Effect of intramuscularly administered polysulphated glycosaminoglycan on articular cartilage from equine joints injected with methylprednisolone acetate. Am J Vet Res 1993; 54: 1359-65.
    PubMedGoogle Scholar
  • 10 Ghosh P, Smith M, Wells C. Second-line agents in osteoarthritis. In Dixon JS, Furst DE. eds. Second-line agents in the treatment of rheumatic diseases.. Marcel Decker Inc.; New York: 1993: 363-427.
    Google Scholar
  • 11 Glade MJ. Polysulphated glycosaminoglycan accelerates net synthesis of collagen and glycosaminoglycans by arthritic cartilage tissues and chondrocytes. Am J Vet Res 1990; 51: 779-85.
    PubMedGoogle Scholar
  • 12 Goodship AE, Silver IA, Wilson AM. Treatment of tendinitis in horses. Vet Rec 1992; 1 (58) 13.
    PubMedGoogle Scholar
  • 13 Hamm D, Goldman L, Jones EW. Polysulphated glycosaminoglycan: a new intraarticular treatment for equine lameness. Vet Med 1984; 6: 811-6.
    PubMedGoogle Scholar
  • 14 Haugland LM, Collier MA, DeBault LE, Walls RC, Flavio Geo de Siqueira L. 3HPSGAG concentration in the synovial fluid of the equine antebrachiocarpal, metacarpophalangeal, coronopedal and tibiotarsal joints following a 500 mg IM injection. J Eq Vet Sci 1995; 15: 274-8.
    CrossrefPubMedGoogle Scholar
  • 15 Howell DS, Carreno MR, Pelletier JP, Muniz OE. Articular cartilage breakdown in a lapine model of osteoarthritis: action of glycosaminoglycan polysulphate ester (GAGPS) on proteoglycan degrading enzyme activity, hexuronate, and cell counts. Clin Orthop 1986; 213: 69-76.
    PubMedGoogle Scholar
  • 16 Howell DS. Muniz OE, Carreno MR. Effect of glycosaminoglycan polysulphate ester on proteoglycan-degrading enzyme activity in an animal model of osteoarthritis. Adv Infl Res 1986; 11: 197-206.
    PubMedGoogle Scholar
  • 17 Kleesiek K, Olschewski G, Schafer N, Greiling H. Influence of antiphlogistic and antidegenerative compounds on the biosynthesis of hyaluronate and proteoglycan. Verh Dtsch Ges Rheumatol 1981; 7: 527-32.
    PubMedGoogle Scholar
  • 18 May SA, Hooke RE, Lees P. The effect of drugs used in the treatment of osteoarthrosis on stromelysin (proteoglycanase) of equine synovial cell origin. Eq Vet J Suppl 1988; 6: 28-32.
    PubMedGoogle Scholar
  • 19 Mikulikova D, Trnavsky K. Influence of a glycosaminoglycan polysulphate (Arteparon ®) on lysosomal enzyme release from human polymorphonuclear leukocytes. Z Rheumatol 1982; 41: 50-3.
    PubMedGoogle Scholar
  • 20 Nishikawa H, Mori I, Umemoto J. Influences of sulphated glycosaminoglycans on biosynthesis of hyaluronic acid in rabbit knee synovial membrane. Arch Biochem Biophys 1985; 240: 146-53.
    CrossrefPubMedGoogle Scholar
  • 21 Oryan A. Experimental tendon injury and repair. PhD Thesis 1989. University of Bristol.;
    Google Scholar
  • 22 Redding WR, Booth LC, Pool RR. The effects of polysulphated glycosaminoglycan on the healing of collagenase induced tendonitis of the equine superficial digital flexor tendon. Vet Surg 1992; 21: 403.
    PubMedGoogle Scholar
  • 23 Smith MM, Ghosh P. The effects of some polysulphated polysaccharides on hyaluronate (HA) synthesis by human synovial fibroblasts. Agents Actions Suppl 1986; 18: 55-62.
    PubMedGoogle Scholar
  • 24 Smith RKW. A case of superficial digital flexor tendinitis: ultrasonographic examination and treatment with intralesional polysulphated glycosaminoglycans. Equine Vet Educ 1992; 4: 280-5.
    CrossrefPubMedGoogle Scholar
  • 25 Steinmeyer J, BurtonWurster N, Lust G. Effects of three antiarthritic drugs on fibronectin and keratin sulphate synthesis by cultured canine articular cartilage chondrocytes. Am J Vet Res 1992; 53: 2077-83.
    PubMedGoogle Scholar
  • 26 Sundqvist H, Forsskahl B, Kvist M. A promising novel therapy for achilles peritendinitis: double-blind comparison of glycosaminoglycan polysulphate and high-dose indomethacin. Int J Sports Med 1987; 8: 298-303.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 27 Todhunter RJ, Freeman KP, Yeager AE, Lust G. Effects of exercise and polysulphated glycosaminoglycan on the development of osteoarthritis in equine carpal joints with osteochondral defects. Vet Surg 1993; 22: 330-42.
    CrossrefPubMedGoogle Scholar
  • 28 Todhunter RJ, Minor RR, Wootton JAM, Krook L, Burton-Wurster N, Lust G. Effects of exercise and polysulphated glycosaminoglycan on repair of articular cartilage defects in the equine carpus. J Orthop Res 1993; 11: 782-95.
    CrossrefPubMedGoogle Scholar
  • 29 Trotter GW, Yovich JV, Mclllwraith CW, Norrdin RW. Effects of intramuscular polysulphated glycosaminoglycan on chemical and physical defects in equine articular cartilage. Can J Vet Res 1989; 53: 224-30.
    PubMedGoogle Scholar
  • 30 Verbruggen G, Veys EM. Influence of sulphated glycosaminoglycans upon proteoglycan metabolism of the synovial lining cells. Acta Rheumatol Belgica 1977; 1: 75-92.
    PubMedGoogle Scholar
  • 31 Yovich JV, Trotter GW, Mcllwraith CW, Norrdin RW. Effects of polysulphated glycosaminoglycan on chemical and physical defects in equine articular cartilage. Am J Vet Res 1987; 48: 1407-13.
    PubMedGoogle Scholar