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Thromb Haemost 1989; 62(03): 1006-1010
DOI: 10.1055/s-0038-1651043
Original Article
Schattauer GmbH Stuttgart

Rat Platelets Adhere to Human Thrombin-Treated Rat Lungs under Flow Conditions

Catherine M Venturini
The Department of Physiology, Albany Medical College, Albany, New York, USA
,
John W Fenton II
*   The Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York, USA
,
Fred L Minnear
The Department of Physiology, Albany Medical College, Albany, New York, USA
,
John E Kaplan
The Department of Physiology, Albany Medical College, Albany, New York, USA
› Author Affiliations
Further Information

Publication History

Received 26 October 1988

Accepted after revision 18 July 1989

Publication Date:
30 June 2018 (online)

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Summary

Platelet attachment to thrombin-treated endothelium was examined under flow conditions in the vascular bed of isolated perfused rat lungs. The infused thrombin bound to the pulmonary endothelium in a specific, competitive manner as shown by displacement of 125I-α-thrombin with an excess of cold diiso-propylfluoro-phosphate-α-thrombin. α-Thrombin significantly increased the attachment of isolated 51Cr-labelled rat platelets to the perfused lungs. Scanning and high voltage electron microscopy showed single platelets in various stages of activation attached to pulmonary endothelium. The receptor binding site and catalytic activity of thrombin were essential to the enhancement of platelet attachment. Aspirin given to rats before the lung isolation had no effect on thrombin-induced platelet attachment. Thus, endothelial bound thrombin initiates platelet activation and enhances subsequent platelet attachment.

Keywords

Platelet adherence - Thrombin - Endothelium Summary
 

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