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Thromb Haemost 1982; 48(03): 253-256
DOI: 10.1055/s-0038-1657273
Original Article
Schattauer GmbH Stuttgart

Inhibition of Activated Coagulation Factor VII by Normal Human Plasma

P E Dahl
The Haematological Laboratory, Medical Department A, Aker Hospital, Oslo, Norway
,
U Abildgaard
The Haematological Laboratory, Medical Department A, Aker Hospital, Oslo, Norway
,
M L Larsen
The Haematological Laboratory, Medical Department A, Aker Hospital, Oslo, Norway
,
L Tjensvoll
The Haematological Laboratory, Medical Department A, Aker Hospital, Oslo, Norway
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 11. Mai 1982

Accepted 06. September 1982

Publikationsdatum:
13. Juli 2018 (online)

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Summary

An amidolytic assay system with tissue thromboplastin (Tpl), purified coagulation factors VII and X, and the chromogenic substrate S-2222 was developed. Antithrombin III (AT) accounts for about one third of the total inhibition exerted by normal plasma in this test system. This effect of AT was prevented by adding purified AT blocking antibodies. Normal plasma and serum showed approximately similar inhibitory effects. The inhibition was probably directed against activated factor VII (F VIIa). Gel filtration of adsorbed normal plasma on Ultrogel AcA 34 showed three inhibitory peaks which were different from AT.

Keywords

Key words Factor VII - Coagulation inhibitors - Chromogenic substrates
 

  • References

  • 1 Hjort PF. Intermediate reactions in the coagulation of blood with tissue thromboplastin. Scand J Clin Lab Invest 1957; 9 (Suppl. 27) 1-183
    CrossrefPubMedSuche in Google Scholar
  • 2 Prydz H, Allison AC. Tissue thromboplastin activity of isolated human monocytes. Thromb Haemostas 1978; 39: 582-591
    PubMedSuche in Google Scholar
  • 3 Aas K. Prokonvertin og konvertin. Thesis. Oslo Akad trykningssentral. 1952: 90
    PubMedSuche in Google Scholar
  • 4 Wagner RH, Brannan jr WM, Brinkhous KM. Antiaccelerator (Anticonvertin) Activity of Canine Plasma and Serum. Proc Soc Exp Biol Med 1955; 89: 266-269
    CrossrefPubMedSuche in Google Scholar
  • 5 Egeberg O. On the Natural Blood Coagulation Inhibitor System – Investigations of Inhibitor Factors Based on Antithrombin Deficient Blood. Thrombos Diathes Haemorrh 1965; 14: 473-489
    PubMedSuche in Google Scholar
  • 6 Godal HC, Rygh M, Laake K. Progressive inactivation of purified factor VII heparin and antithrombin III. Throinb Res 1974; 5: 773-775
    PubMedSuche in Google Scholar
  • 7 Østerud B, Miller-Andersson M, Abildgaard U, Prydz H. The Effect of Antithrombin III on the Activity of the Coagulation Factors VII, IX and X. Thromb Haemostas 1976; 35: 295-304
    PubMedSuche in Google Scholar
  • 8 Harboe N, Ingild A. Immunization Isolation of Immunglobulins, Estimation of Antibody Titre. Scand J Immunol 1933; 2 (Suppl. 01) 161
    PubMedSuche in Google Scholar
  • 9 Hvatum M, Prydz H. Studies on tissue thromboplastin I. Solubilization with deoxycholate. Biochim Biophys Acta 1966; 130: 92-101
    CrossrefPubMedSuche in Google Scholar
  • 10 Laake K, Ellingsen R. Purification and some characteristics of factor VII in human titrated plasma, glass-activated serum, and cold-activated plasma. Thromb Res 1974; 5: 539-556
    CrossrefPubMedSuche in Google Scholar
  • 11 Aurell L, Friberger P. Determination of Factor X in plasma with S-2222. Laboratory Instruction. Kabi Diagnostica. 1977
    PubMedSuche in Google Scholar
  • 12 Gladhaug A, Prydz H. Purification of the coagulation factors VII and X from human serum. Some properties of factor VII. Biochim Biophys Acta 1970; 215: 105-111
    CrossrefPubMedSuche in Google Scholar
  • 13 Abildgaard U, Lie M, Ødegård OR. Antithrombin (heparin cofactor) assay with “new” chromogenic substrates (S-2238 and Chromozym-TH). Thromb Res 1977; 11: 749-553
    PubMedSuche in Google Scholar
  • 14 Nemerson Y. Biological Control of Factor VII. Thromb Haemostas 1976; 35: 96-100
    PubMedSuche in Google Scholar
  • 15 Hermansky F, Vitek J. The Role of Proconvertin and Stuart Factor in the Inactivation of Tissue Thromboplastin by Serum. Experientia 1960; 16: 455-456
    CrossrefPubMedSuche in Google Scholar
  • 16 Seligsohn U, Østerud B, Rapaport SI. Coupled Amidolytic Assay for Factor VII: Its Use with a Clotting Assay to Determine the Activity State of Factor VII. Blood 1978; 52 (05) 978-988
    PubMedSuche in Google Scholar
  • 17 Østerud B, Rapaport SI. Activation of Factor IX by the Reaction Product of Tissue Factor and Factor VII: Additional pathway for initiating blood coagulation. Proc Natl Acad Sci USA 1977; 74: 5260-5264
    CrossrefPubMedSuche in Google Scholar