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Planta Med 2015; 81(03): 215-221
DOI: 10.1055/s-0034-1396204
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Downregulation of Microglial Activation by Achillolide A

Anat Elmann
1   Department of Food Quality and Safety, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
,
Alona Telerman
1   Department of Food Quality and Safety, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
,
Sharon Mordechay
1   Department of Food Quality and Safety, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
2   Faculty of Agriculture, Food and Environmental Quality Sciences of the Hebrew University of Jerusalem, Rehovot, Israel
,
Hilla Erlank
1   Department of Food Quality and Safety, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
2   Faculty of Agriculture, Food and Environmental Quality Sciences of the Hebrew University of Jerusalem, Rehovot, Israel
,
Miriam Rindner
1   Department of Food Quality and Safety, Volcani Center, Agricultural Research Organization, Bet Dagan, Israel
,
Yoel Kashman
3   School of Chemistry, Tel Aviv University, Ramat Aviv, Israel
,
Rivka Ofir
4   Dead Sea & Arava Science Center and Regenerative Medicine & Stem Cell Research Center, Ben-Gurion University of the Negev, Beer-Sheba, Israel
› Author Affiliations
Further Information

Publication History

received 06 March 2014
revised 03 December 2014

accepted 09 December 2014

Publication Date:
05 February 2015 (online)

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Abstract

Chronic inflammation has been implicated in the pathogenesis of various neurodegenerative diseases. During the neuroinflammatory process, microglial cells release neurotoxic and proinflammatory mediators. In the present study, using activity-guided fractionation, we have purified an anti-inflammatory compound determined by spectroscopic methods to be a sesquiterpene lactone named achillolide A from Achillea fragrantissima (Forsk.) Sch. Bip. In primary cultures of lipopolysaccharide-activated microglial cells, achillolide A inhibited the lipopolysaccharide-induced levels of proinflammatory and toxic mediators including glutamate, nitric oxide, matrix metalloproteinase-9, cyclooxygenase-2, induced nitric oxide synthase, interleukin-1β, and tumor necrosis factor-α. Achillolide A also exhibited an antioxidant capacity, as was shown in a cell free system as well as by its ability to reduce intracellular reactive oxygen species levels in microglial cells. Thus, achillolide A might have therapeutic potential for treatment of neurodegenerative diseases and deserves further studies.

Key words

Achillea fragrantissima - Asteraceae - achillolide A - microglial cells - inflammation - neurodegenerative diseases

Supporting Information

 

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