Inhibitory Constituents of Euscaphis japonica on Lipopolysaccharide-Induced Nitric Oxide Production in BV2 Microglia

Abstract

Bioactivity-guided fractionation of the methanolic extract of the aerial parts of Euscaphis japonica (Tunb.) Kantiz (Staphyleaceae), afforded a new compound, p-coumaroyl-D-malic acid 1-methyl ester (1), together with twelve known compounds, 3,7-dihydro-5-octanolide (2), blumenol A (3), megastigmane (4), gallic acid (5), stenophyllin H1 (6), methyl 5,7-dihydroxyoctanoate (7), trans-phytol (8), α-tocopherol (9), kaempferol (10), kaempferol 3-O-β-D-glucopyranoside (11), quercetin (12) and quercetin 3-O-β-D-glucopyranoside (13). Among them, compounds 1 - 5 and 8 - 13 significantly inhibited lipopolysacchride-induced nitric oxide production in murine BV2 microglial cells. Especially, compounds 5, 8, 10 and 12 exerted potent inhibitory activity comparable to that of NAME, used as positive control.

Key words

Euscaphis japonica - p-coumaroyl-D-malic acid 1-methyl ester - Staphyleaceae - nitric oxide - BV2 microglia - anti-inflammatory

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