Important Role of TNF-α in Inhibitory Effects of Radix Sophorae Flavescentis Extract on Vascular Restenosis in a Rat Carotid Model of Balloon Dilatation Injury

Abstract

The aim of this study was to determine the molecular mechanism underlying the inhibition of vascular restenosis by Radix Sophorae Flavescentis (RSF) extract after balloon dilatation injury. In a rat carotid model of balloon dilatation injury, the RSF extract showed a significant inhibitory effect on vascular restenosis. Immunohistochemistry showed that the expression of tumor necrosis factor-α (TNF-α) in the vascular focus was markedly reduced upon treatment with the RSF extract, whereas the expression of proliferating cell nuclear antigens (PCNA) was mostly unaffected. Colorimetric assays of methylene blue incorporation demonstrated that the proliferation of cultured vascular smooth muscle cells (VSMCs) was not inhibited with serum from rats treated with RSF extract. However, the expression of TNF-α in cultured VSMCs was significantly downregulated by serum from rats treated with RSF extract. These results suggested that RSF extract has an inhibitory effect on vascular restenosis after balloon dilatation injury which might be, in part, attributable to its inhibition of TNF-α expression.

Key words

Radix Sophorae Flavescentis - Sophora flavescens Ait. - Fabaceae - vascular restenosis - TNF‐α - balloon dilatation injury

References

• 1 Li Y, Yang Y, Fang L, Zhang Z, Jin J, Zhang K. Anti-hepatitis activities in the broth of Ganoderma lucidum supplemented with a Chinese herbal medicine.  Am J Chin Med. 2006;  34 341-349
• 2 Lau F Y, Chui C H, Gambari R. Antiproliferative and apoptosis-inducing activity of Brucea javanica extract on human carcinoma cells.  Int J Mol Med. 2005;  16 1157-1162
• 3 Hu Z L, Zhang J P, Yu X B, Lin W, Qian D H, Wan M B. Effect of matrine on ipopolysaccharides/D-galactosamine-induced hepatitis and tumor necrosis factor release from macrophages in vitro.  Acta Pharmacol Sin. 1996;  174 351-353
• 4 Lee S W, Lee H S, Nam J Y, Kwon O E, Baek J A, Chang J S, Rho M C, Kim Y K. Kurarinone isolated from Sophora flavescens Ait inhibited MCP‐1-induced chemotaxis.  J Ethnopharmacol. 2005;  97 515-519
• 5 Gaspardone A, Versaci F. Coronary stenting and inflammation.  Am J Cardiol. 2005;  96 65L-70L
• 6 Miller A M, McPaden A R, Preston A, Wadsworth R M, Wainwright C L. TNF-α increases the inflammatory response to vascular balloon injury without accelerating neointimal formation.  Atherosclerosis. 2005;  179 51-59
• 7 Shebuski R J, Kilgore K S. Role of inflammatory mediators in thrombogenesis.  J Pharmacol Exp Ther. 2002;  300 729-735
• 8 Warner S JC, Libby P. Human vascular smooth muscle cells: target for and source of tumor necrosis factor.  J Immunol. 1989;  142 100-109
• 9 Tanaka H, Sukhova G, Schwartz D, Libby P. Proliferating arterial smooth muscle cells after balloon injury express TNF-α but not interleukin-1 or basic fibroblast growth factor.  Arterioscler Thromb Vasc Biol. 1996;  16 1218-1223
• 10 Wang X, Sun W, Sun H, Lv H, Wu Z, Wang P, Liu L, Cao H. Analysis of the constituents in the rat plasma after oral administration of Yin Chen Hao Tang by UPLC/Q‐TOF‐MS/MS.  J Pharm Biomed Anal. 2008;  46 477-490
• 11 Yang D, Cai S, Liu H, Guo X, Li C, Shang M, Wang X, Zhao Y. On-line identification of the constituents of Buyang Huanwu decoction in pig serum using combined HPLC‐DAD‐MS techniques.  J Chromatogr B Analyt Technol Biomed Life Sci. 2006;  831 288-302
• 12 Matsuura K, Kawakita T, Nakai S, Saito Y, Suzuki A, Nomoto K. Role of B-lymphocytes in the immunopharmacological effects of a traditional Chinese medicine, xiao-chai-hu-tang (shosaiko-to).  Int Immunopharmacol. 1993;  15 237-243
• 13 Umeda M, Amagaya S, Ogihara Y. Effects of certain herbal medicines on the biotransformation of arachidonic acid: a new pharmacological testing method using serum.  J Ethnopharmacol. 1988;  23 91-98
• 14 Indolfi C, Esposito G, Di Lorenzo E, Rapacciuolo A, Feliciello A, Porcellini A, Avvedimento V E, Condorelli M, Chiariello M. Smooth muscle cell proliferation is proportional to the degree of balloon injury in a rat model of angioplasty.  Circulation. 1995;  92 1230-1235
• 15 [no authors listed]. Pharmacopoeia of People's Republic of China. Beijing; Chemical Industry Publishing House 2005: 141
• 16 Ross R, Glomset J, Kariya B, Harker L. A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro.  Proc Natl Acad Sci USA. 1974;  71 1207-1210
• 17 López-Franco O, Suzuki Y, Sanjuán G, Blanco J, Hernández-Vargas P, Yo Y, Kopp J, Egido J, Gómez-Guerrero C. Nuclear factor-kappa B inhibitors as potential novel anti-inflammatory agents for the treatment of immune glomerulonephritis.  Am J Pathol. 2002;  161 1497-1505
• 18 Galis Z S, Sukhova G K, Lark M W, Libby P. Increased expression of matrix metalloproteinases and matrix degrading activity in vulnerable regions of human atherosclerotic plaques.  J Clin Invest. 1994;  94 2493-2503
• 19 Monraats P S, Pires N M, Schepers A, Agema W R, Boesten L S, de Vries M R, Zwinderman A H, de Maat M P, Doevendans P A, de Winter R J, Tio R A, Waltenberger J, 't Hart L M, Frants R R, Quax P H, van Vlijmen B J, Havekes L M, van der Laarse A, van der Wall E E, Jukema J W. Tumor necrosis factor-alpha plays an important role in restenosis development.  FASEB J. 2005;  19 1998-2004
• 20 Jovinge S, Hultgårdh-Nilsson A, Regnstrošm J, Nilsson J. Tumor necrosis factor-alpha activates smooth muscle cell migration in culture and is expressed in the balloon-injured rat aorta.  Arterioscler Thromb Vasc Biol. 1997;  17 490-497
• 21 Takeda R, Suzuki E, Satonaka H, Oba S, Nishimatsu H, Omata M, Fujita T, Nagai R, Hirata Y. Blockade of endogenous cytokines mitigates neointimal formation in obese Zucker rats.  Circulation. 2005;  111 1398-1406
• 22 Tan B, Huang J F, Wei Q, Zhang H, Ni R Z. Anti-hepatoma effect and its mechanism of arsenic trioxide on experimental liver cancer.  World Chin J Digestol. 2004;  12 32-37
• 23 Ling J Y, Zhang G Y, Cui Z J, Zhang C K. Supercritical fluid extraction of quinolizidine alkaloids from Sophora flavescens Ait. and purification by high-speed counter-current chromatography.  J Chromatogr A. 2007;  1145 123-127
• 24 Ma L, Wen S, Zhan Y, He Y, Liu X, Jiang J. Anticancer effects of the Chinese medicine matrine on murine hepatocellular carcinoma cells.  Planta Med. 2008;  74 245-251
• 25 Joner M, Finn A V, Farb A, Mont E K, Kolodgie F D, Ladich E, Kutys R, Skorija K, Gold H K, Virmani R. Pathology of drug-eluting stents in humans: delayed healing and late thrombotic risk.  J Am Coll Cardiol. 2006;  48 193-202