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Planta Med 2015; 81(02): 123-129
DOI: 10.1055/s-0034-1383409
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Identification of Angiotensin-Converting Enzyme Inhibitory Proteins from Mycelium of Pleurotus pulmonarius (Oyster Mushroom)

Badjie Xietaqieuallah Ibadallah
1   Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
,
Noorlidah Abdullah
1   Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia
,
Adawiyah Suriza Shuib
2   University of Malaya Centre for Proteomics Research, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia
› Author Affiliations
Further Information

Publication History

received 24 May 2014
revised 09 November 2014

accepted 22 November 2014

Publication Date:
15 January 2015 (online)

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Abstract

Pleurotus pulmonarius (grey oyster mushroom) has been acknowledged as a recuperative agent for many diseases in addition to its recognition as a nutritious provision. We performed a study on P. pulmonarius mycelium for an antihypertensive effect via the angiotensin-converting enzyme inhibitory activity. The preliminary assay on the mycelial water extract demonstrated that the angiotensin-converting enzyme inhibitory activity had an IC50 value of 720 µg/mL. Further protein purifications via ammonium sulphate precipitation and RP-HPLC resulted in 60× stronger angiotensin-converting enzyme inhibitory activity than that of the mycelial water extract (IC50 = 12 µg/mL). Protein identification and characterisation by MALDI-TOF/TOF, later corroborated by LC-MS/MS, indicated three proteins that are responsible for the blood pressure lowering effects via different mechanisms: serine proteinase inhibitor-like protein, nitrite reductase-like protein, and DEAD/DEAH box RNA helicase-like protein.

Key words

ACE inhibitor - bioactive protein - hypertension - fungi - Pleurotaceae - Pleurotus pulmonarius - oyster mushroom
 

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