Identification of Botanicals and Potential Contaminants through RFLP and Sequencing

Michelle R. Lum; Ellie Potter; Thanh Dang; David Heber; Mary Hardy; Ann M. Hirsch

doi:10.1055/s-2005-871230

Planta Medica, Inhaltsverzeichnis

Planta Med 2005; 71(9): 841-846
DOI: 10.1055/s-2005-871230

Original Paper

Biochemistry and Molecular Biology
© Georg Thieme Verlag KG Stuttgart · New York

Identification of Botanicals and Potential Contaminants through RFLP and Sequencing

Michelle R. Lum¹ ^, ⁴ , Ellie Potter¹ ^, ⁴ , Thanh Dang¹ , David Heber² , Mary Hardy² , Ann M. Hirsch¹ ^, ³

¹Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA
²Center for Human Nutrition, School of Medicine, University of California, Los Angeles, CA, USA
³Molecular Biology Institute, University of California, Los Angeles, CA, USA
⁴These authors should both be considered jointly as first author

Abstract

Botanical supplements for health enhancement are being increasingly used in the United States, but no safeguards are formally in place to ensure that they are not contaminated with non-efficacious or potentially harmful plant material. A molecular approach, which allows the authentication of botanical ingredients and detection of contaminating plant material by analyzing the ITS-1 region by PCR-RFLP and subsequent sequencing, is described. When using starting material from which DNA can be obtained, this method has the potential for identifying both primary and contaminating plant material in botanical dietary supplements.

Key words

PCR amplification - alfalfa - red clover - repair reaction - RFLP - dietary supplements

Volltext

Referenzen

References

1 Slifman N R, Obermeyer W R, Aloi B K, Musser S M, Correll W A, Cichowicz S M. et al . Contamination of botanical dietary supplements by Digitalis lanata . New Eng J Med. 1998; 339 806-11
2 Vanherweghem J -L, Depierreux M; Tielemans C, Abramowicz D, Dratwa M, Jadoul M. et al . Rapidly progressive interstitial fibrosis in young women: association with slimming regimen including Chinese herbs. Lancet. 1993; 341 387-91
3 Vanherweghem J -L. Misuse of herbal remedies: the case of an outbreak of terminal renal failure in Belgium (Chinese herbs nephropathy.) . J Altern Complement Med. 1998; 4 9-13
4 Frasca T, Brett A S, Yoo S D. Mandrake toxicity. A case of mistaken identity. Arch Intern Med. 1997; 157 2007-9
5 Quality control methods for medicinal plant materials. Geneva; World Health Organization 1998
6 Brisken D P, Leroy A, Gawienowski M E. Influence of nitrogen on the production of hypericins by St. John’s wort. Plant Physiol Biochem. 2000; 38 1-8
7 Pusch C M, Giddings I, Scholz M. Repair of degraded duplex DNA from prehistoric samples using Escherichia coli DNA polymerase I and T4 DNA ligase. Nucleic Acids Res. 1998; 26 857-9
8 LeRoy A, Potter E, Woo H -Y, Heber D, Hirsch A M. Characterization and identification of alfalfa and red clover dietary supplements using a PCR-based method. J Agric Food Chem. 2002; 50 5063-9
9 Baldwin B, Sanderson M, Porter J, Wojciechowski M, Campbell C, Donoghue M. The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann Missouri Bot Garden. 1995; 82 247-277
10 Blattner F R. Direct amplification of the entire ITS region from poorly preserved plant material using recombinant PCR. Biotechniques. 1999; 27 1180-6
11 Altschul S F, Gish W, Miller W, Myers E W, Lipman D J. Basic local alignment search tool. J Mol Biol. 1990; 215 403-10
12 Thompson J D, Higgins D G, Gibson T J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22 4673-80
13 Mihalov J J, Marderosian A D, Pierce J C. DNA identification of commercial ginseng samples. J Agric Food Chem. 2000; 48 3744-52
14 Zhang K TB, Leung H W, Yeung H W, Wong R NS. Differentiation of Lycium barbarum from its related Lycium species using random amplified polymorphic DNA. Planta Med. 2001; 67 379-81
15 Cheng K T, Tsay H S, Chen C F, Chou T W. Determination of the components in a Chinese prescription, Yu-Ping-Feng San, by RAPD analysis. Planta Med. 1998; 64 563-5
16 Ha W -Y, Yau C -F, Shaw P -C, Wang J. Differentiation of Panax ginseng from P. quinquefolius by Amplified Fragment Length Polymorphism. In: Shaw PC, Wang J, But PP-H, editors Authentication of Chinese Medicinal Materials by DNA Technology. Singapore; World Scientific 2002: pp 109-24
17 Ngan F, Shaw P, But P, Wang J. Molecular authentication of Panax species. Phytochemistry. 1999; 50 787-91
18 Fu R -Z, Wang J, Zhang Y -B, Wang Z -T, But P P-H, Li N. et al . Differentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphism. Planta Med. 1999; 65 648-50
19 Hillis D, Dixon M. Ribosomal DNA: Molecular evolution and phylogenetic inference. Q Rev Biol. 1991; 66 411-53

Ann M. Hirsch

Department of Molecular, Cell and Developmental Biology

University of California

Los Angeles

CA 90095-1606

USA

Telefon: +1-310-206-8673

Fax: +1-310-206-5413

eMail: ahirsch@ucla.edu