Cytotoxic Cardenolides from the Leaves of Acokanthera oblongifolia

Łukasz Pecio; Emad M. Hassan; Elsayed A. Omer; Gabriela Gajek; Renata Kontek; Andrzej Sobieraj; Anna Stochmal; Wiesław Oleszek

doi:10.1055/a-0958-2566

Planta Medica, Inhaltsverzeichnis

Planta Med 2019; 85(11/12): 965-972
DOI: 10.1055/a-0958-2566

Biological and Pharmacological Activities

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Cytotoxic Cardenolides from the Leaves of Acokanthera oblongifolia ^{[
¹
]}

Autor*innen

Łukasz Pecio

¹Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation State Research Institute, Puławy, Poland
Emad M. Hassan

²Medicinal and Aromatic Plants Research Department, National Research Centre, Giza, Egypt
Elsayed A. Omer

²Medicinal and Aromatic Plants Research Department, National Research Centre, Giza, Egypt
Gabriela Gajek

³Laboratory of Cytogenetics, Department of Molecular Biotechnology and Genetics, University of Lodz, Poland
Renata Kontek

³Laboratory of Cytogenetics, Department of Molecular Biotechnology and Genetics, University of Lodz, Poland
Andrzej Sobieraj

⁴Department of Neurology and Neurosurgery, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland
Anna Stochmal

¹Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation State Research Institute, Puławy, Poland
Wiesław Oleszek

¹Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation State Research Institute, Puławy, Poland

Abstract

Three previously undescribed cardenolides, acovenosigenin A 3-O-α-L-acofriopyranoside (1), 14-anhydroacovenosigenin A 3-O-[β-D-glucopyranosyl-(1″→4′)-O-α-L-acofriopyranoside] (2), and 14-anhydroacovenosigenin A 3-O-[β-D-glucopyranosyl-(1″→4′)-O-α-L-acovenopyranoside] (3), together with the two already known ones, 14-anhydrodigitoxigenin 3-O-β-D-glucopyranoside (4) and acospectoside A (5), were isolated from the leaves of Acokanthera oblongifolia. The influence of cardenolides 1 – 3 and acovenoside A (found in the Acokanthera genus) on three cancer cell lines (HT29, HCT116, and AGS) was also investigated. The most promising results, in comparison with oxaliplatin, were obtained for compound 1, which was found to be highly cytotoxic for all tested cell lines, HT29 (IC₅₀ = 63.49 nM), HCT116 (IC₅₀ = 67.35 nM), and AGS (IC₅₀ = 80.92 nM). Unfortunately, 1 also showed similar toxicity towards normal lymphocytes (IC₅₀ = 98.03 nM).

Key words

Acokanthera oblongifolia - cardenolide - cancer - cytotoxicity - Apocynaceae

Volltext

Referenzen

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Cytotoxic Cardenolides from the Leaves of Acokanthera oblongifolia [ 1 ]

Autor*innen

Cytotoxic Cardenolides from the Leaves of Acokanthera oblongifolia ^{[
¹
]}