Planta Med 2020; 86(01): 78-84
DOI: 10.1055/a-1019-9401
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

A Compound Isolated from Phyllanthus tenellus Demonstrates Metabolic and Vascular Effects In Vitro

Omar Estrada
1  Centre of Biophysics and Biochemistry, Venezuelan Institute for Scientific Research, Caracas, Venezuela
,
Camilo Di Giulio
1  Centre of Biophysics and Biochemistry, Venezuelan Institute for Scientific Research, Caracas, Venezuela
,
Radharani Dorta-Ledezma
1  Centre of Biophysics and Biochemistry, Venezuelan Institute for Scientific Research, Caracas, Venezuela
,
Freddy Gonzalez-Mujica
2  Faculty of Medicine, Central University of Venezuela, Caracas, Venezuela
,
Norma Motta
2  Faculty of Medicine, Central University of Venezuela, Caracas, Venezuela
,
Elsa Zea
2  Faculty of Medicine, Central University of Venezuela, Caracas, Venezuela
,
Nelson Cupitra
3  Physiology and Biochemistry Research group-PHYSIS, Faculty of Medicine, University of Antioquia, Medellín, Colombia
,
Whendy Contreras
1  Centre of Biophysics and Biochemistry, Venezuelan Institute for Scientific Research, Caracas, Venezuela
,
Raúl Narvaez-Sanchez
3  Physiology and Biochemistry Research group-PHYSIS, Faculty of Medicine, University of Antioquia, Medellín, Colombia
,
Juan C. Calderón
3  Physiology and Biochemistry Research group-PHYSIS, Faculty of Medicine, University of Antioquia, Medellín, Colombia
› Author Affiliations
Further Information

Publication History

received 18 May 2019
revised 23 September 2019

accepted 26 September 2019

Publication Date:
25 October 2019 (online)

Abstract

Common chronic conditions such as metabolic syndrome and diabetes are increasingly associated to metabolic and cardiovascular complications. Although Phyllanthus tenellus leaves have been used in decoctions as a popular remedy to control blood glucose levels and hypertension, its use needs a scientific basis. This study was therefore undertaken to report a phytochemical analysis of P. tenellus leaves and to test if the main active compound has potential to simultaneously tackle several pathophysiological features of metabolic syndrome and diabetes-related metabolic and vascular disorders such as hyperglycaemia, increased platelet activation, and endothelial dysfunction. We performed a partition of the methanolic extract of P. tenellus leaves among different organic solvents followed by chromatographic separation guided by the rat liver microsomal glucose-6-phosphatase assay. Two known tannins were identified by spectroscopic methods as pinocembrin-7-O-[3″-O-galloyl-4″,6″-(S)-hexahydroxydiphenoyl]-α-D-glucose, named P7OG by us, and gemin D. The structural determination of the isolated compounds was based on spectral data. The ability of the main active component, P7OG, to inhibit human platelet aggregation and to modify vascular reactivity of rat aortic rings incubated with high glucose (D-glucose 55 mM) was then evaluated. P7OG was further able to inhibit platelet aggregation induced by adenosine 5′-diphosphate and collagen, showed vasorelaxant effects in arteries precontracted with phenylephrine, and reverted the endothelium-dependent impairment effect of high glucose in rat aortic rings. In conclusion, one tannin isolated from P. tenellus showed promising metabolic, antiaggregant, and vascular effects, which suggests the potential beneficial use of P. tenellus to tackle complex cardiometabolic diseases.

Supporting Information