Planta Med 2020; 86(04): 255-266
DOI: 10.1055/a-1087-7405
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Patchouli Oil Attenuates High Fat Diet-induced Non-alcoholic Hepatic Steatosis

Nan Xu
1   Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China
,
Xue Wu
1   Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China
,
Hui-Juan Luo
1   Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China
,
Fang-Fang Xu
3   The Second Clinical College of Guangzhou University of Chinese Medicine Guangzhou, Guangzhou University of Chinese Medicine, China
,
Qiong-Hui Huang
1   Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China
,
Jia-Zhen Wu
1   Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China
,
Yu-Xuan Gan
1   Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China
,
Min-Yao Li
1   Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China
,
Zheng-Quan Lai
2   Department of Pharmacy, Shenzhen University General Hospital, Shenzhen University, Shenzhen, China
,
Hui-Ran Lin
5   Laboratory Animal Management Office, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China
,
Jian-Nan Chen
1   Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China
,
Zi-Ren Su
1   Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China
4   Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan, China
,
Wei-Peng Ai
2   Department of Pharmacy, Shenzhen University General Hospital, Shenzhen University, Shenzhen, China
,
Yu-Hong Liu
1   Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China
› Author Affiliations
Supported by: Characteristic Cultivation Program for Subject Research of Guangzhou University of Chinese Medicine XKP2019007
Supported by: Key Program for Subject Research of Guangzhou University of Chinese Medicine XK2019002
Supported by: Guangdong Basic and Applied Basic Research Foundation 2019A1515010815
Supported by: Natural Science Foundation of Shenzhen University General Hospital SUGH2018QD035 & SUGH2018QD070
Supported by: Traditional Chinese Medicine Bereau of Guangdong Province No.20181121
Supported by: Youth Innovative Talents Project of Colleges and Universities of Guangdong Province 2018KQNCX216
Supported by: Key Disciplines Construction Projects of High-level University of Guangdong Province, Specific Research Project of Science and Technology of Guangdong Provincial Hospital of TCM No.YN2016QJ05
Further Information

Publication History

received 26 July 2019
revised 09 December 2019

accepted 23 December 2019

Publication Date:
23 January 2020 (online)


Abstract

Non-alcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases worldwide. Nevertheless, no first-line therapy exists. Hepatic steatosis is the earliest stage of NAFLD, which is characterized by an accumulation of hepatic lipids. Patchouli oil (PO), which is isolated from the well-known Chinese herb named Pogostemon cablin (Blanco) Benth. (Lamiaceae), inhibits hepatic lipid accumulation effectively. However, its potential ability for the treatment of NAFLD had not been reported before. Thus, the objective of this study was to investigate the effectiveness of PO against hepatic steatosis and its underlying mechanisms. We used a high fat diet (HFD)-induced hepatic steatosis model of rats to estimate the effect of PO against NAFLD. Hematoxylin-eosin and oil red O staining were used to analyze the hepatic histopathological changes. ELISA, RT-qPCR, and Western blotting analysis were applied to evaluate the parameters for hepatic steatosis. Our results showed that PO significantly attenuated the lipid profiles and the serum enzymes, evidenced by quantitative and histopathological analyses. It also markedly down-regulated the expression of sterol regulatory element-binding protein 1 (SREPB-1c) with its downstream factors in de novo lipogenesis. And, likewise, in lipid export by very low-density lipoproteins (VLDL), related molecules were dramatically improved. Furthermore, PO observably normalized the aberrant peroxisome proliferator-activated receptor α (PPAR-α) signal in fatty acids oxidation. In conclusion, PO exerted a preventing effect against HFD-induced steatosis and might be due to decrease de novo lipogenesis, promote export of lipids, as well as owing to improve fatty acids oxidation.

Supporting Information

 
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