Planta Med 2020; 86(16): 1225-1234
DOI: 10.1055/a-1201-3375
Biological and Pharmacological Activity
Original Papers

Chemical Characterization of Lavandula dentata Essential Oil Cultivated in Chile and Its Antibiofilm Effect against Candida albicans

Andrea Müller-Sepúlveda
1   Institute for Research in Dental Sciences (ICOD), Faculty of Dentistry, University of Chile, Santiago, Chile
2   Institute of Agrifood, Animals and Environmental Sciences, Universidad de OʼHiggins, Rancagua, Chile
Camila Cid Chevecich
1   Institute for Research in Dental Sciences (ICOD), Faculty of Dentistry, University of Chile, Santiago, Chile
José A. Jara
1   Institute for Research in Dental Sciences (ICOD), Faculty of Dentistry, University of Chile, Santiago, Chile
Carolina Belmar
1   Institute for Research in Dental Sciences (ICOD), Faculty of Dentistry, University of Chile, Santiago, Chile
Pablo Sandoval
1   Institute for Research in Dental Sciences (ICOD), Faculty of Dentistry, University of Chile, Santiago, Chile
Rocío Santander Meyer
3   Departament of Environmental Sciences, Faculty of Chemistry and Biology, Universidad de Santiago de Chile, Santiago, Chile
Raúl Quijada
4   Faculty of Physical and Mathematical Sciences, Universidad de Chile, Santiago, Chile
Sidnei Moura
5   Laboratory of Biotechnology of Natural and Synthetic Products, Biotechnology Institute, Universidade de Caixas do Sul, Caixas do Sul, Brazil
Rodrigo López-Muñoz
6   Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile
Mario Díaz-Dosque
1   Institute for Research in Dental Sciences (ICOD), Faculty of Dentistry, University of Chile, Santiago, Chile
1   Institute for Research in Dental Sciences (ICOD), Faculty of Dentistry, University of Chile, Santiago, Chile
› Author Affiliations
Supported by: Fundación para la Innovación Agraria FIA PYT-2017-0853
Supported by: Universidad de O´higgins postdoctoral fellowship 0056/2019
Supported by: Vicerrectoría de Investigación y Desarrollo, Universidad de Chile Proyecto Enlace ENL32/18


Candida albicans is the most common human fungal pathogen, and with the increase in resistance rates worldwide, it is necessary to search for new pharmacological alternatives. Lavandula dentata L. essential oil is recognized as having antimicrobial properties. However, its effect against fungal biofilms has been poorly described. C. albicans-related infections involve the development of biofilms, which are highly resistant to conventional antifungals. In this work, we evaluated the antibiofilm effect of L. dentata L. essential oil against C. albicans. First, we characterized the essential oil by gas chromatography-mass spectrometry. The antifungal effect on C. albicans reference strains was evaluated by a disk diffusion assay and the minimal inhibitory concentration was obtained through a microdilution assay. The effect of the essential oil on the adhesion ability of C. albicans was determined through a crystal violet assay, and morphogenesis inhibition was assessed by light microscopy. The effect of the essential oil on the microarchitecture of biofilms was evaluated through scanning electron microscopy. Finally, the antibiofilm effect was evaluated through an adapted biofilm scratch assay and XTT viability assay. The main constituent of the essential oil was the monoterpenoid eucalyptol (60%). The essential oil presented minimal inhibitory concentrations of 156 and 130 µg/mL against two strains assayed. This minimal inhibitory concentration inhibited adhesion, morphogenesis, biofilm formation, altered microarchitecture, and decreased the viability of established biofilms formed on abiotic surfaces for both strains assayed. This study demonstrates that the essential oil from L. dentata could be a promising treatment against C. albicans biofilms.

Supporting Information

Publication History

Received: 23 January 2020

Accepted after revision: 09 June 2020

Article published online:
14 July 2020

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