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Planta Med 2021; 87(01/02): 101-112
DOI: 10.1055/a-1320-4556
Natural Product Chemistry and Analytical Studies
Original Papers

Time-Scale Shifting of Volatile Semiochemical Levels in Wild Type Lychnophora ericoides (Brazilian arnica) and Pollinator Records

Daniel Petinatti Pavarini
1   Núcleo de Pesquisas em Produtos Naturais e Sintéticos, Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil
,
João Semir   †,*
2   Instituto de Biologia, Departamento de Botânica, Universidade Estadual de Campinas, Campinas, SP, Brazil
,
João Luís Callegari Lopes
1   Núcleo de Pesquisas em Produtos Naturais e Sintéticos, Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil
,
Ricardo Roberto da Silva
1   Núcleo de Pesquisas em Produtos Naturais e Sintéticos, Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil
,
Norberto Peporine Lopes 
1   Núcleo de Pesquisas em Produtos Naturais e Sintéticos, Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil
› Author AffiliationsSupported by: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Supported by: Conselho Nacional de Desenvolvimento Científico e Tecnológico
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo
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Abstract

Lychnophora ericoides is a Brazilian folk phytomedicine from Cerradoʼs “campus rupestris”. Its volatile organic compounds includes bisabolene-derivatives as major compounds. Herein we provide the chemical profiling of constitutive volatile sesquiterpenes from L. ericoides leaves, timeframe emissions surveys, and pollinators records. In situ samples of L. ericoides were harvested. A headspace-solid phase micro extraction method of pre-concentration was optimized. Identification was done through GC-MS. Isolation and structural elucidation were performed whenever necessary. Pollinators were registered in pictures and video. Short time-series and harmonic regressions determined rhythms of single compounds, and average chromatographic signal area was used to determine mono and sesquiterpene rhythms. Concluding, optimized headspace-solid phase micro extraction method of terpenes level analysis was reached. α-Pinene, β-pinene, α-terpinene, para-cymene, limonene, γ-terpinene, terpinen-4-ol, dehydro-sesquicineole, and β-guaiene were identified using GC-MS data. 11-dehydro cadinol and ortho-acetoxy bisabolol were elucidated. Sesquiterpenes concentrations were higher due to temperature rise, lower leaf age, and flowering seasons. Harmonic regressions determined that daylight might control levels of terpenes. Hummingbird, hemiptera insects, and wasps were recorded visiting Compositae capitulum for the first time. We studied nondomestic plants from in situ conditions and concluded that bisabolene-derivative levels were more abundant than monoterpenes during flowering throughout the summer.

Key words

Compositae - chemical profiling - headspace-SPME and GC-MS - Lychnophora ericoides - neotropical flora - pollinators - terpene emissions

†,* in memoriam


Supporting Information

    Over 45 items, including tables, figures, equations, and spectra are available online.

    Concerning the data availability, the datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.
    Figs: Authors attest that all photographs and the video (also available at youtube.com/watch?v=lVNOMdOoIlU) herein presented are original from our field activities and were taken/recorded by Daniel P. Pavarini.

  • Supporting Information


Publication History

Received: 15 June 2020

Accepted: 20 November 2020

Article published online:
04 December 2020

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
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