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Planta Med 2023; 89(11): 1074-1086
DOI: 10.1055/a-1858-6898
Natural Product Chemistry and Analytical Studies
Original Papers

Microstructured Polymer System Containing Proanthocyanidin-Enriched Extract from Limonium brasiliense as a Prophylaxis Strategy to Prevent Recurrence of Porphyromonas gingivalis

Fernanda Pilatti 
1   Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maringá, Maringá, Brazil
3   Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany
,
Raquel Isolani 
1   Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maringá, Maringá, Brazil
,
Larissa Valone 
1   Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maringá, Maringá, Brazil
,
Mariana Nascimento de Paula 
1   Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maringá, Maringá, Brazil
,
Angelo de Oliveira Caleare 
1   Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maringá, Maringá, Brazil
,
Sabrina Barbosa de Souza Ferreira 
1   Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maringá, Maringá, Brazil
,
Marcos Luciano Bruschi 
1   Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maringá, Maringá, Brazil
,
Daniela Cristina de Medeiros Araújo 
1   Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maringá, Maringá, Brazil
,
Terezinha Aparecida Guedes 
2   Department of Statistics, Universidade Estadual de Maringá, Maringá, Brazil
,
Andreas Hensel 
3   Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany
,
João Carlos Palazzo de Mello 
1   Postgraduate Program in Pharmaceutical Sciences, Palafito, Universidade Estadual de Maringá, Maringá, Brazil
› Author AffiliationsSupported by: Conselho Nacional de Desenvolvimento Científico e Tecnológico 200175/2018-1
Supported by: Conselho Nacional de Desenvolvimento Científico e Tecnológico 401490/2014-9
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Abstract

Periodontal diseases are a global oral health problem affecting almost 10% of the global population. Porphyromonas gingivalis is one of the main bacteria involved in the initiation and progression of inflammatory processes as a result of the action of the cysteine proteases lysin- and arginine-gingipain. Surelease/polycarbophil microparticles containing a lyophilized proanthocyanidin-enriched fraction from the rhizomes of Limonium brasiliense, traditionally named “baicuru” (ethyl acetate fraction), were manufactured. The ethyl acetate fraction was characterized by UHPLC by the presence of samarangenins A and B (12.10 ± 0.07 and 21.05 ± 0.44%, respectively) and epigallocatechin-3-O-gallate (13.44 ± 0.27%). Physiochemical aspects of Surelease/polycarbophil microparticles were characterized concerning particle size, zeta potential, entrapment efficiency, ethyl acetate fraction release, and mucoadhesion. Additionally, the presence of the ethyl acetate fraction-loaded microparticles was performed concerning potential influence on viability of human buccal KB cells, P. gingivalis adhesion to KB cells, gingipain activity, and P. gingivalis biofilm formation. In general, all Surelease/polycarbophil microparticles tested showed strong adhesion to porcine cheek mucosa (93.1 ± 4.2% in a 30-min test), associated with a prolonged release of the ethyl acetate fraction (up to 16.5 ± 0.8% in 24 h). Preincubation of KB cells with Surelease/polycarbophil microparticles (25 µg/mL) resulted in an up to 93 ± 2% reduced infection rate by P. gingivalis. Decreased activity of the P. gingivalis-specific virulence factors lysin- and arginine-gingipain proteases by Surelease/polycarbophil microparticles was confirmed. Surelease/polycarbophil microparticles decreased biofilm formation of P. gingivalis (97 ± 2% at 60 µg/mL). Results from this study prove the promising activity of Surelease/polycarbophil microparticles containing ethyl acetate fraction microparticles as a prophylaxis strategy to prevent the recurrence of P. gingivalis.

Key words

Limonium brasiliense - Plumbaginaceae - adhesion - gingipain - microparticles - Porphyromonas gingivalis

Supporting Information

    The results of cell viability, the coincubation assay, and the 8-h release test are available as Supporting Information.

  • Supporting Information


Publication History

Received: 22 February 2022

Accepted after revision: 18 May 2022

Accepted Manuscript online:
22 May 2022

Article published online:
28 September 2022

© 2022. Thieme. All rights reserved.

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Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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