Planta Med 2023; 89(03): 333-346
DOI: 10.1055/a-1942-5504
Pharmacokinetic Investigations
Original Papers

Metabolite Profiling of Swertia cincta Extract in Rats and Pharmacokinetics Study of Three Bioactive Compounds Using UHPLC-MS/MS

Xiaoyin Ye
2   School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China
Tong Zhang
1   Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China
2   School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China
Han Han
1   Experiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China
2   School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China
› Author Affiliations
Supported by: National Scientific and Technological Major Special Project of China 2019ZX09201004-002
Supported by: Natural Science Foundation of Shanghai 21ZR1460500
Supported by: National Natural Science Foundation of China 82173946


Swertia cincta, a plant of the genus Swertia in Gentianceae, has “heat-clearing” and detoxifying effects that normalize the gallbladder function in the treatment of jaundice. Although numerous studies on Swertia cincta have been performed, the absorption and pharmacokinetic behaviors remain unclear. In this study, the compounds of Swertia cincta in serum, bile, feces, and urine of rats were analyzed using a ultra-high-performance liquid chromatography-tandem mass spectrometry. A total of 9 prototype components and 48 metabolites were detected in biological samples. Furthermore, we determined the main components absorbed in the blood of Swertia cincta and established a method for simultaneously determining these components (sweroside, swertiamarin, and gentiopicroside) in positive ionization mode within 6 min. The quantitative method was successfully applied for the multiple-component pharmacokinetic study of Swertia cincta.

Supporting Information

Publication History

Received: 16 May 2022

Accepted after revision: 13 September 2022

Accepted Manuscript online:
13 September 2022

Article published online:
20 November 2022

© 2022. Thieme. All rights reserved.

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