Planta Med
DOI: 10.1055/a-1956-7829
Original Papers

Five new diarylbutyrolactones and sesquilignans from Saussurea medusa and their inhibitory effects on LPS-induced NO production

JingYa Cao
1   Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining, China (Ringgold ID: RIN34721)
2   University of the Chinese Academy of Sciences, Beijing, China (Ringgold ID: RIN74519)
,
Zhiyao Wang
3   Henan Academy of Science, Zhengzhou Henan, China
,
J. Stewart Alan
4   School of Medicine, University of St Andrews, St Andrews, United Kingdom of Great Britain and Northern Ireland
,
Qi Dong
1   Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining, China (Ringgold ID: RIN34721)
,
Ye Zhao
5   Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, China (Ringgold ID: RIN58298)
,
Lijuan Mei
1   Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining, China (Ringgold ID: RIN34721)
,
Yanduo Tao
1   Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining, China (Ringgold ID: RIN34721)
,
Rui Tao Yu
6   Qinghai Provincial Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology Chinese Academy of Sciences, Xining, China (Ringgold ID: RIN34721)
› Author Affiliations
Supported by: science and innovation platform for the development and construction of special project of Key Laboratory of Tibetan Medicine Research of Qinghai Province 2022-ZJ-Y03
Supported by: Natural Science Foundation of Qinghai Province 2022-ZJ-930

Five new diarylbutyrolactones and sesquilignans (1a/1b−4), including one pair of enantiomers (1a/1b), together with ten known analogues (5−14), were isolated from the whole plants of Saussurea medusa. Compound 1 was found to possess an unusual 7,8'-diarylbutyrolactone lignan structure. Separation by chiral HPLC analysis led to the isolation of one pair of enantiomers, (+)-1a and (−)-1b. The structures of the new compounds were elucidated by extensive spectroscopic data. All compounds, except compounds 5, 7 and 9, were isolated from S. medusa for the first time. Moreover, compounds 1−4, 8 and 10−14 had never been obtained from the genus Saussurea previously. Compounds (+)-1a, 2, 5, 7, and 9−11 were found to inhibit the lipopolysaccharide (LPS)-induced release of NO by RAW264.7 cells with IC50 values ranging from 10.1 ± 1.8 to 41.7 ± 2.1 μM. Molecular docking and iNOS expression experiments were performed to examine the interactions between the active compounds and the iNOS enzyme.



Publication History

Received: 04 June 2022

Accepted after revision: 06 October 2022

Accepted Manuscript online:
06 October 2022

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