Planta Med 2023; 89(15): 1457-1467
DOI: 10.1055/a-2148-7163
Biological and Pharmacological Activity
Original Papers

Structural Characterization and Anticomplement Activity of an Acidic Heteropolysaccharide from Lysimachia christinae Hance

Zhou Hong
1   State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China
2   School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, China
Li-Shuang Zhou
2   School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, China
2   School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, China
Guo-Qi Yuan
2   School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, China
Xiao-Jiang Wang
2   School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, China
Yan Lu
2   School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, China
Dao-Feng Chen
1   State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, China
2   School of Pharmacy, Institutes of Integrative Medicine, Fudan University, Shanghai, China
› Author Affiliations
This work was supported by grants from the National Natural Science Foundation of China (No 81872977), National Key R&D Program of China (No 2019YFC1711000), and Development Project of Shanghai Peak Disciplines-Integrative Medicine (No 20180101).


A novel acidic heteropolysaccharide (LCP-90-1) was isolated and purified from a traditional “heat-clearing” Chinese medicine, Lysimachia christinae Hance. LCP-90-1 (Mw, 20.65 kDa) was composed of Man, Rha, GlcA, Glc, Gal, and Ara, with relative molar ratios of 1.00: 3.00: 11.62: 1.31: 1.64: 5.24. The backbone consisted of 1,4-α-D-GlcpA, 1,4-α-D-Glcp, 1,4-β-L-Rhap, and 1,3,5-α-L-Araf, with three branches of β-D-Galp-(1 → 4)-β-L-Rhap-(1→, α-L-Araf-(1→ and α-D-Manp-(1→ attached to the C-5 position of 1,3,5-α-L-Araf. LCP-90-1 exhibited potent anticomplement activity (CH50: 135.01 ± 0.68 µg/mL) in vitro, which was significantly enhanced with increased glucuronic acid (GlcA) content in its degradation production (LCP-90-1-A, CH50: 28.26 ± 0.39 µg/mL). However, both LCP-90-1 and LCP90-1-A were inactivated after reduction or complete acid hydrolysis. These observations indicated the important role of GlcA in LCP-90-1 and associated derivatives with respect to anticomplement activity. Similarly, compared with LCP-90-1, the antioxidant activity of LCP-90-1-A was also enhanced. Thus, polysaccharides with a high content of GlcA might be important and effective substances of L. christinae.

Supporting Information

Publication History

Received: 27 April 2023

Accepted after revision: 04 August 2023

Accepted Manuscript online:
04 August 2023

Article published online:
23 August 2023

© 2023. Thieme. All rights reserved.

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