Chemical Structural Elucidation and Immunomodulatory Activity of a New Polysaccharide from Saposhnikoviae Radix

Yifang Cui; Haitao Fan; Meng Sun; Xinyang He; Jie Li; Guangzhong Tu; Yanyan Jiang; Bin Liu

doi:10.1055/a-2573-7718

Planta Medica, Table of Contents

Planta Med 2025; 91(09): 513-523
DOI: 10.1055/a-2573-7718

Original Papers

Chemical Structural Elucidation and Immunomodulatory Activity of a New Polysaccharide from Saposhnikoviae Radix

Yifang Cui‡

¹School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China

,

Haitao Fan‡

²College of Bioengineering, Beijing Polytechnic, Beijing, China

,

Meng Sun

¹School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China

,

Xinyang He

¹School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China

,

Jie Li

¹School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China

,

Guangzhong Tu

³Beijing Institute of Microchemistry, Beijing, China

,

Yanyan Jiang

¹School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China

⁴The Key Research Laboratory of “Exploring Effective Substance in Classic and Famous Prescriptions of Traditional Chinese Medicine”, The State Administration of Traditional Chinese Medicine of the Peopleʼs Republic of China, Beijing, China

,

Bin Liu

¹School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China

⁴The Key Research Laboratory of “Exploring Effective Substance in Classic and Famous Prescriptions of Traditional Chinese Medicine”, The State Administration of Traditional Chinese Medicine of the Peopleʼs Republic of China, Beijing, China

› Author Affiliations

Abstract

The chemical structure and immunomodulatory activity of a new homogeneous polysaccharide, SP4002501, isolated from Saposhnikoviae Radix (SR), were investigated. Purification of SP4002501 was performed by DEAE-Cellulose and Sepharose CL-6B column chromatography. The monosaccharidic constituents were identified as rhamnose (Rha), galacturonic acid (GalA), galactose (Gal), and arabinose (Ara) with a molar ratio of 3.7: 86.6: 2.7: 7.1. According to the methylation analysis, partial hydrolysis, FT-IR, and NMR analysis, SP4002501 had a backbone of polygalacturonic acid units with a small amount of galactose (Gal). Side chains are connected to C-3 of galactose (Gal) and consist of rhamnose (Rha), galacturonic acid (GalA), galactose (Gal), and arabinose (Ara), with arabinose (Ara) as terminal sugar. Biological activity assessment suggests that SP4002501 exhibits immunomodulatory activity through promoting macrophage proliferation and phagocytosis.

Keywords

polysaccharide - Saposhnikoviae Radix - Saposhnikovia divaricata - Apiaceae - chemical structure - immunomodulatory activity

Full Text

References

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