Alkaloidgehalte in haploiden und diploiden Blättern und Zellsuspensionen von Datura innoxia

Abstract

Comparative investigations on alkaloid content of leaves and suspension cultures (fermenter batch cultures) of haploid and diploid Datura innoxia Mill, indicated a higher content of tropane alkaloids in the leaves of diploid plants than in haploids, but approximately the same content in suspension cultures of both ploidy levels. In both systems and in all strains tested, scopolamin was the major alkaloid, however total alkaloid content in cell suspensions was far below that detected in leaves of the same strains. No correlation was found in the quantitative ratio of scopolamin and hyoscyamin between leaves and fermenter cultures of cell suspensions of the same strain and between various strains. Furthermore, cultured suspensions are able to excrete considerable amounts of scopolamin and hyoscyamin into the culture medium. Our results indicate that the synthesis of the major tropan alkaloids is not restricted to an organogenetic status in haploid and diploid Datura innoxia material.

Key Word Index

Datura innoxia - Fermenter–Batch–Culture - Ploidy Levels - Tropanalkaloids