STEROIDSAPONINE UND STEROIDSAPOGENINE

Abstract

From the overground part of Tribulus terrestris L. a saponine mixture was isolated in a quantity of 2,8 % containing 10 steroid substances (from A to K). A small admixture of phenol was also proved (fig. 1).

With the help of repeated column chromatography and thin-layer chromatography the saponines C, F and G were isolated (Fig. 2).

Saponine F proved to be a new product: tigogenine–3–diglucorhamnoside, named by us terrestroside F, the partial structure of which was determined on the basis of the hydrolytic products: aglykon tigogenine (identified by m. p., mixed m. p., IR– and mass spectrum, acetyl derivative) and an oligosaccharide part – rhamnose : glucose (2:1).

The saponines C and G proved to be a mixture of two tigogenine and diosgenine glycosides each. The mixture of aglykons was separated by column chromatography on silica gel containing silver nitrate and identified by the above mentioned indices as tigogenine and diosgenine (Table I). In the hydrolysates the sugars glucose and rhamnose were proved.

A flavonoide was also isolated which was identified as astragaline (caempferol–3–glucoside).