Planta Med 2009; 75(5): 557-560
DOI: 10.1055/s-0029-1185321
Biochemistry, Molecular Biology and Biotechnology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Authentication of Panax ginseng from its Adulterants by PCR-RFLP and ARMS

Ying Diao1 , 2 , Xian-Ming Lin3 , Chao-Lin Liao3 , Chun-Zi Tang3 , Zhong-Jian Chen4 , Zhong-Li Hu1
  • 1Key Lab of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan, P. R. China
  • 2College of Life Science and Technology, Chongqing University of Arts and Sciences, Chongqing, P. R. China
  • 3Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi, P. R. China
  • 4Wenshan Sanqi Research Institute, Wenshan, P. R. China
Further Information

Publication History

received January 4, 2008 revised November 14, 2008

accepted December 3, 2008

Publication Date:
02 February 2009 (eFirst)

Abstract

As a widely used and expensive herbal medicine, Panax ginseng has many adulterants in the commercial market. PCR-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system (ARMS) based on 5S rDNA sequence analysis were applied to identify two common adulterants of P. ginseng. The sizes of 5S rRNA gene non-transcribed spacers (NTS) sequences in P. ginseng and its adulterants were determined, ranging from 143 to 424 bp. The PCR product of P. ginseng only could be digested among the tested specimens because of its specific SpeI restriction site found in the 5S rDNA sequence. In addition, P. ginseng was successfully identified from compound medicinal preparations and from the Single-Taste medicines. These results suggest that the methods are able to authenticate P. ginseng.

References

Prof. Dr. Zhong-Li Hu

Key Lab of the Ministry of Education for Plant Developmental Biology
College of Life Science
Wuhan University

Wuhan 430072

People's Republic of China

Phone: + 86 (0) 27 68 75 36 06

Fax: + 86 (0) 27 68 75 36 11

Email: huzhongli@whu.edu.cn