Planta Med 2009; 75(11): 1221-1226
DOI: 10.1055/s-0029-1185536
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Bioassay-Guided Isolation of Anti-Inflammatory C-Glucosylflavones from Passiflora edulis

Silvana Maria Zucolotto1 , Stella Goulart2 , Ana B. Montanher2 , Flávio Henrique Reginatto1 , Eloir Paulo Schenkel1 , Tânia Silvia Fröde2
  • 1Department of Pharmaceutical Science, Center of Health Sciences, Federal University of Santa Catarina, Campus, Universitário – Trindade, Florianópolis, SC, Brazil
  • 2Department of Clinical Analysis, Center of Health Sciences, Federal University of Santa Catarina, Campus Universitário – Trindade, Florianópolis, SC, Brazil
Further Information

Publication History

received Sept. 9, 2008 revised February 25, 2009

accepted March 3, 2009

Publication Date:
07 April 2009 (online)

Abstract

Passiflora edulis, commonly known as “maracujá”, is widely cultivated in Brazil for the industrial production of juice. The species of Passiflora are popularly used as a sedative or tranquillizer, and also against intermittent fever and skin inflammation. In this study we evaluated the anti-inflammatory activity of four sub-fractions and three isolated compounds from the butanolic fraction of P. edulis var. flavicarpa leaves, using the mouse model of pleurisy induced by carrageenan. The butanolic fraction obtained from an aqueous extract of P. edulis (50 and 100 mg/kg, i. p.) showed anti-inflammatory activity by inhibiting leukocytes and neutrophils (p < 0.01). Sub-fraction C showed itself to be more effective than the other sub-fractions (p < 0.01). Isoorientin (1), vicenin-2 (2) and spinosin (3) were isolated from the active sub-fraction C derived from the butanolic fraction. The sub-fraction C (50 mg/kg, i. p.), as well as its major isolated compounds (25 mg/kg, i. p.), inhibited leukocytes and neutrophils (p < 0.05). Additionally, the butanolic fraction and isoorientin also inhibited myeloperoxidase activity (p < 0.05). The present study showed that the C-glucosylflavones isolated from P. edulis leaves can be responsible for the anti-inflammatory effect of P. edulis on the mouse model of pleurisy.

References

Tânia Sílvia Fröde

Department of Clinical Analysis
Center of Health Sciences
Federal University of Santa Catarina (UFSC)
Campus Universitário

Trindade, s/n

Florianópolis, CEP 88040-970

Santa Catarina

Brazil

Phone: + 55 48 37 21 98 56

Fax: + 55 48 32 44 09 36

Email: [email protected]