Planta Med 2009; 75 - PJ110
DOI: 10.1055/s-0029-1234915

Isolation and purification of new minor dihydropyranochromone and furanocoumarin from fruits of Peucedanum alsaticum L. by high-speed counter-current chromatography

K Skalica-Woźniak 1, T Mroczek 1, I Garrard 2, K Głowniak 1
  • 1Department of Pharmacognosy with Medicinal Plant Laboratory, Medicinal University of Lublin, 1 Chodzki Str., 20–093 Lublin, Poland
  • 2Advanced Bioprocessing Centre, Brunel Institute for Bioengineering, Brunel University, Uxbridge UB8 3PH, UK

Genus Peucedanum is a large group comprising more than 120 species, widely distributed in Europe, Asia and Africa. Peucedanum alsaticum L. is species occurring naturally in Poland but also in central and eastern Europe. However the chemical composition of examined plant is poorly known, although some flavonoids, coumarins (peucedanin, imperatorin), phenolic acids and essential oils have been investigated [1–6].

A preparative high-speed counter-current chromatography (HSCCC) method was successfully used for isolation of two new minor compounds – namely alsaticol (dihydropyranochromone) and alsaticocoumarin A (5-substituted furanocoumarin) – never identified in nature before. Compounds were obtained from dichloromethane extract of Peucedanum alsaticum fruits and their identification was performed with NMR and MS methods. This is first time when these kind of natural compounds, occurring as minor constituents, were isolated using high-speed counter-current chromatography method (HSCCC) with application of preparative 900ml coil. This development makes an opportunity towards large scale isolation of these novel minor constituents from P. alsaticum or other plant species abundant in furanocoumarins and pyranochromones.

Acknowledgment: This work was financially supported by grant no. N N405 3513 33 from the Polish Ministry of Science and Higher Education.

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[6] Skalicka-Wożniak, K. (2008) Chromatographia 68:85–90.