Abstract
Using exhaustive chromatographic separation we have isolated (−)-tigloyl-deangeloyl-gomisin
F as a novel dibenzocyclooctadiene lignan from Schisandra chinensis. With the help of HPLC, we further isolated (+)-schisandrin, (+)-deoxyschisandrin,
(+)-γ-schisandrin, (−)-gomisin J, (+)-gomisin A, (−)-gomisin N, (−)-tigloyl-gomisin
P, (−)-wuweizisu C, (−)-gomisin D, rubrisandrin A, (−)-gomisin G, (+)-gomisin K3 and (−)-schisantherin C. A full NMR description of (−)-schisantherin C was carried
out with the aim to confirm previous reports of its structure. Compounds isolated
were identified on the basis of UV, IR, 1H- and 13C‐NMR and MS. The cytotoxicity of lignans was tested for the BY-2 cell line alone
and as a synergistic effect with the cytotoxic agent camptothecin. Lignans showed
various toxicity and synergistic and antagonistic effects on camptothecin-induced
cytotoxicity. Cytotoxicity against colon cancer cell line LoVo was also tested.
Key words
Schisandra chinensis
- Schisandraceae - dibenzocyclooctadiene lignans - cytotoxicity
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Karel Šmejkal
Department of Natural Drugs
Faculty of Pharmacy
University of Veterinary and Pharmaceutical Sciences Brno
Palackého 1–3
61242 Brno
Czech Republic
Telefon: + 42 05 41 56 28 39
eMail: karel.mejkal@post.cz