Planta Med 2011; 77(2): 128-132
DOI: 10.1055/s-0030-1250159
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Comparative X‐Ray and Conformational Analysis of a New Crystal of 13α,21-Dihydroeurycomanone with Eurycomanone from Eurycoma longifolia and Their Anti-Estrogenic Activity Using the Uterotrophic Assay

Chin-Hoe Teh1 , Mahfoudh Abdulghani1 , Hiroshi Morita3 , Motoo Shiro4 , Abas Hj Hussin2 , Kit-Lam Chan1
  • 1School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia
  • 2Center for Drug Research, Universiti Sains Malaysia, Penang, Malaysia
  • 3Faculty of Pharmaceutical Sciences, Hoshi University, Shinagawa-ku, Tokyo, Japan
  • 4X‐ray Research Laboratory, Rigaku Corporation, Akishima, Tokyo, Japan
Further Information

Publication History

received Nov. 23, 2009 revised June 23, 2010

accepted June 26, 2010

Publication Date:
21 July 2010 (online)


13α,21-Dihydroeurycomanone (1), a known quassinoid of Eurycoma longifolia Jack was recrystallized from chloroform into a novel crystal structure in space group P21. Its X‐ray data were compared with those of eurycomanone (2). Following intraperioneal injections at similar doses of 2.44 µmol/kg/day for 3 consecutive days, 2 displayed comparable potency with tamoxifen but was more potent than 1 in the anti-estrogenic effect against 17α-ethynylestradiol (EE)-induced uterotrophy of immature rats.


Prof. Dr. Kit-Lam Chan

School of Pharmaceutical Sciences
Universiti Sains Malaysia

11800 Penang


Phone: + 60 46 53 26 96

Fax: + 60 46 57 68 36