Planta Med 2011; 77(4): 388-393
DOI: 10.1055/s-0030-1250353
Analytical Studies
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Chromatographic Fingerprint and Quantitative Analysis of Seven Bioactive Compounds of Scutellaria barbata

Hong Yao1 , Shaoguang Li1 , Juan Hu2 , Yan Chen1 , Liying Huang1 , Jianhua Lin3 , Guangwen Li1 , Xinhua Lin1
  • 1Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou Fujian, P. R. China
  • 2Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou Fujian, P. R. China
  • 3Department of Orthopedics, the First Affiliated Hospital of Fujian Medical University, Fuzhou Fujian, P. R. China
Further Information

Publication History

received July 9, 2010

accepted August 20, 2010

Publication Date:
22 September 2010 (online)


Scutellaria barbata D. Don is widely used as a folk antitumor and anti-inflammatory agent in Asia. However, a simple and global quality control method for S. barbata was lacking. In this study, six phenolic compounds, including p-coumaric acid, scutellarin, apigenin 5-O-β-glucopyranoside, luteolin, apigenin and 4′-hydroxywogonin were obtained from S. barbata by phytochemical investigations. The six compounds plus baicalein show cytotoxcities to the nine human cancer cells, K562, MGC-803, HL60, SH-SY5Y, SW1116, SMMC-7221, SW480, HepG2 and KB. Subsequently, a high-performance liquid chromatography with photodiode array detector (HPLC‐DAD) was developed for both fingerprint analysis of S. barbata and quantitative determination of the seven anticancer active compounds in S. barbata. The chromatographic separation was accomplished on an Ultimate™ XB‐C18 column (4.6 × 250 mm, 5 µm) in 65 min. For fingerprinting, 26 common peaks were found and selected as characteristic peaks to assess the consistency of S. barbata samples. For quantitative analysis, the seven bioactive compounds showed good regression relationship (R 2 > 0.999) within test ranges and the recovery of the method was in the range of 90–105 %. In brief, the present study provides the fingerprint analysis and quantitative methods for global and systematical quality control of S. barbata for its anticancer usage.


Prof. Jianhua Lin

The First Affiliated Hospital of Fujian Medical University

Department of Orthopedics


Fujian 350005

People's Republic of China

Phone: +86 5 91 83 35 71 99

Fax: +86 5 91 83 31 87 16


Prof. Xinhua Lin

Department of Pharmaceutical Analysis
Faculty of Pharmacy
Fujian Medical University

Fuzhou 350004

People's Republic of China

Phone: +86 5 91 22 86 20 16

Fax: +86 5 91 22 86 20 16