Planta Med 2011; 77(4): 368-373
DOI: 10.1055/s-0030-1250362
Natural Product Chemistry
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Diterpene Alkaloids from Aconitum anthora and Assessment of the hERG-Inhibiting Ability of Aconitum Alkaloids

Peter Forgo1 , Botond Borcsa1 , Dezső Csupor1 , László Fodor2 , Róbert Berkecz3 , Attila Molnár V.4 , Judit Hohmann1
  • 1Department of Pharmacognosy, University of Szeged, Szeged, Hungary
  • 2Gedeon Richter Plc., Budapest, Hungary
  • 3Department of Medical Chemistry, University of Szeged, Szeged, Hungary
  • 4Department of Botany, University of Debrecen, Debrecen, Hungary
Further Information

Publication History

received May 19, 2010 revised August 19, 2010

accepted August 25, 2010

Publication Date:
22 September 2010 (online)

Abstract

A new norditerpene alkaloid, 10-hydroxy-8-O-methyltalatizamine (1), was isolated from the whole plant of Aconitum anthora L. besides the known isotalatizidine (2) and hetisinone (3). The structures were determined by means of HR-ESI‐MS, 1D and 2D NMR spectroscopy, including 1H-1H COSY, NOESY, HSQC and HMBC experiments, resulting in complete 1H and 13C chemical shift assignments for 13, and revision of some earlier 13C‐NMR data. The effects of the isolated compounds, together with twenty-one other Aconitum alkaloids with different skeletal types and substitution patterns, on hERG channels were studied by the whole-cell patch clamp technique, using the QPatch-16 automated patch clamp system. At 10 µM, aconitine, 14-benzoylaconine 8-O-palmitate, songoramine, gigactonine and neolinine demonstrated significant hERG K+ channel inhibition; all other compounds exerted only low (6–21 %) inhibitory activity.

References

Prof. Judit Hohmann

Department of Pharmacognosy
University of Szeged

Eotvos u. 6

6720 Szeged

Hungary

Phone: +36 62 54 64 53

Fax: +36 62 54 57 04

Email: hohmann@pharm.u-szeged.hu